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Real-time PCR assay for diagnosis of eukaryotic peptide chain release factor subunit 1 gene in the stages of giardia duodenalis
Joint Authors
Mulla, Siba Fadil Ali
Hamzah, Damia Makki
Source
Journal of Babylon University : Journal of Applied and Pure Sciences
Issue
Vol. 24, Issue 5 (31 Dec. 2016), pp.1234-1245, 12 p.
Publisher
Publication Date
2016-12-31
Country of Publication
Iraq
No. of Pages
12
Main Subjects
Abstract EN
Giardiasis caused by Giardia duodenalis is a common intestinal disease.
Although this parasitic infection is found in mammals including human, pets and livestock.
A total of 250 samples was collected from patients that were suspected the infection with Giardia duodenalis during the period from August 2014 to October 2015.
The aim of this study is to investigate the molecular identification of Giardia duodenalis in patients suffered from diarrhea depending on a Eukaryotic peptide chain release factor subunit 1 gene to Confirm its existence in both stages of Giardia dudenalis (Trophozoit and ) and the possibility adopting it as a diagnostic gene.
The results revealed that 200 samples were positive depending on the microscopic examination , 100 samples of stool have been chosen to isolate the cysts from it, while 100 samples of stool were used for trophozoits isolation , which in turn have undergone the third stage of the examination for the purpose of qualitative accurate diagnosis of Giardia using of quantitative Real Time PCR assay for amplifying the target region Eukaryotic peptide chain release factor subunit 1 and study the possibility of using the a diagnostic gene.
American Psychological Association (APA)
Mulla, Siba Fadil Ali& Hamzah, Damia Makki. 2016. Real-time PCR assay for diagnosis of eukaryotic peptide chain release factor subunit 1 gene in the stages of giardia duodenalis. Journal of Babylon University : Journal of Applied and Pure Sciences،Vol. 24, no. 5, pp.1234-1245.
https://search.emarefa.net/detail/BIM-698069
Modern Language Association (MLA)
Mulla, Siba Fadil Ali& Hamzah, Damia Makki. Real-time PCR assay for diagnosis of eukaryotic peptide chain release factor subunit 1 gene in the stages of giardia duodenalis. Journal of Babylon University : Journal of Applied and Pure Sciences Vol. 24, no. 5 (2016), pp.1234-1245.
https://search.emarefa.net/detail/BIM-698069
American Medical Association (AMA)
Mulla, Siba Fadil Ali& Hamzah, Damia Makki. Real-time PCR assay for diagnosis of eukaryotic peptide chain release factor subunit 1 gene in the stages of giardia duodenalis. Journal of Babylon University : Journal of Applied and Pure Sciences. 2016. Vol. 24, no. 5, pp.1234-1245.
https://search.emarefa.net/detail/BIM-698069
Data Type
Journal Articles
Language
English
Notes
Text in English ; abstracts in English and Arabic.
Record ID
BIM-698069