Local study of blaCTX-M genes detection in Proteus spp. by using PCR technique
Other Title(s)
دراسة محلية للتحري عن جينات blaCTX-M في جنس بكتيريا المتقلبات Proteus spp. باستخدام تقنية تفاعل الكوثر
Author
Source
Issue
Vol. 57, Issue 2C (30 Jun. 2016), pp.1371-1376, 6 p.
Publisher
University of Baghdad College of Science
Publication Date
2016-06-30
Country of Publication
Iraq
No. of Pages
6
Main Subjects
Topics
Abstract EN
In this study, 25 clinical isolates of Proteus spp.
were collected from urine, wounds and burns specimens from different hospitals in Baghdad city, all isolates were identified by using different bacteriological media, biochemical assays and Vitek-2 system.
It was found that 15 (60 %) isolates were identifies as Proteus mirabilis and 10 (40 %) isolates were Proteus vulgaris.
The susceptibility of P.
mirabilis and P.
vulgaris isolates towards cefotaxime was (66.6 %) and (44.4 %) respectively; while the susceptibility of P.
mirabilis and P.
vulgaris isolates towards ceftazidime was (20 %).
Extended spectrum β-lactamses producing Proteus was (30.7 %).
DNA of 10 isolates of P.
mirabilis and 4 isolates of P.
vulgaris were extracted and detection of (blaCTX-M-1, blaCTX-M-2, blaCTX-M-8 and blaCTX-M-9) was done by multiplex polymerase chain reaction (PCR).
Results showed the presence of blaCTX-M-2 gene, which is responsible for resistance to cefotaxime in these isolates, while no other types of , blaCTX-M genes were found in them.
American Psychological Association (APA)
Passat, Daymah Nazar Faraj. 2016. Local study of blaCTX-M genes detection in Proteus spp. by using PCR technique. Iraqi Journal of Science،Vol. 57, no. 2C, pp.1371-1376.
https://search.emarefa.net/detail/BIM-699554
Modern Language Association (MLA)
Passat, Daymah Nazar Faraj. Local study of blaCTX-M genes detection in Proteus spp. by using PCR technique. Iraqi Journal of Science Vol. 57, no. 2C (2016), pp.1371-1376.
https://search.emarefa.net/detail/BIM-699554
American Medical Association (AMA)
Passat, Daymah Nazar Faraj. Local study of blaCTX-M genes detection in Proteus spp. by using PCR technique. Iraqi Journal of Science. 2016. Vol. 57, no. 2C, pp.1371-1376.
https://search.emarefa.net/detail/BIM-699554
Data Type
Journal Articles
Language
English
Notes
Text in English ; abstracts in English and Arabic.
Record ID
BIM-699554