Hypnotic effect of Ocimum basilicum on pentobarbital-induced sleep in mice
Joint Authors
Askari, Vahid Rida
Rahimi, Vafa Baradaran
Ghurbani, Ahmad
Rakhshandeh, Hassan
Source
Iranian Red Crescent Medical Journal
Issue
Vol. 18, Issue 7 (31 Jul. 2016), pp.1-6, 6 p.
Publisher
Publication Date
2016-07-31
Country of Publication
United Arab Emirates
No. of Pages
6
Main Subjects
Abstract EN
Background : Sleep disorders are accompanied by several complications, and currently used soporific drugs can induce unwanted effects such as psychomotor impairment, tolerance, amnesia, and rebound insomnia.
Objectives : The present study was carried out to investigate if Ocimum basilicum has a sleep-prolonging effect.
Materials and Methods : This work was an experimental study on 72 mice which were randomly divided into 9 groups: saline (control); diazepam (3 mg/kg, positive control); hydro-alcoholic extract (HAE) of Ocimum basilicum (25, 50, or 100 mg / kg); ethyl acetate fraction (EAF, 50 mg / kg); n-butanol fraction (NBF, 50 mg / kg); water fraction (WF, 50 mg / kg); and saline containing 10 % DMSO (vehicle for EAF and NBF).
All the test compounds were injected intraperitoneally (IP) 30 minutes before pentobarbital administration (30 mg/kg).
Duration and latency of pentobarbital-induced sleep were recorded.
Also, LD50 of HAE was determined and the cytotoxicity of HAE was tested on neural and fibroblast cells using the MTT assay.
Results: HAE increased the duration of pentobarbital-induced sleep at doses of 25, 50, and 100 mg/kg (P < 0.001).
The hypnotic effect of HAE was comparable to that induced by diazepam.
Similarly, WF, EAF, and NBF at 50 mg/kg could increase sleep duration.
The sleep latency was decreased by HAE (P < 0.01 - P < 0.001) and NBF (P < 0.001), but not by WF and EAF.
The LD50 value for HAE was found to be 2.4 g/kg.
HAE had no effect on the viability of neuronal PC12 cells and L929 fibroblast cells.
Conclusions : The present data demonstrated that Ocimum basilicum potentiates sleeping behaviors without any cytotoxicity.
The main component (s) responsible for the hypnotic effects of this plant is most likely a non-polar agent (s) which is found in NBF.
Isolation of the active constituents may yield a novel sedative drug.
American Psychological Association (APA)
Askari, Vahid Rida& Rahimi, Vafa Baradaran& Ghurbani, Ahmad& Rakhshandeh, Hassan. 2016. Hypnotic effect of Ocimum basilicum on pentobarbital-induced sleep in mice. Iranian Red Crescent Medical Journal،Vol. 18, no. 7, pp.1-6.
https://search.emarefa.net/detail/BIM-701421
Modern Language Association (MLA)
Rakhshandeh, Hassan…[et al.]. Hypnotic effect of Ocimum basilicum on pentobarbital-induced sleep in mice. Iranian Red Crescent Medical Journal Vol. 18, no. 7 (Jul. 2016), pp.1-6.
https://search.emarefa.net/detail/BIM-701421
American Medical Association (AMA)
Askari, Vahid Rida& Rahimi, Vafa Baradaran& Ghurbani, Ahmad& Rakhshandeh, Hassan. Hypnotic effect of Ocimum basilicum on pentobarbital-induced sleep in mice. Iranian Red Crescent Medical Journal. 2016. Vol. 18, no. 7, pp.1-6.
https://search.emarefa.net/detail/BIM-701421
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 6
Record ID
BIM-701421