Use box -pcr to study genetic relatedness of some pseuaomonaus aeruginosa isolates from different clinieal and environmental sourees In Baghdad
Other Title(s)
استعمال BOX-PCR لدراسة التقارب الوراثي لبعض من بكتريا pseudomonaus aeruginosa المعزولة من مصادر مرضية و بيئية مختلفة في بغداد
Source
Journal of the College of Basic Education
Issue
Vol. 19, Issue 80 (31 Dec. 2013), pp.723-732, 10 p.
Publisher
al-Mustansiriyah University College of Basic Education
Publication Date
2013-12-31
Country of Publication
Iraq
No. of Pages
10
Main Subjects
Abstract EN
totally twenty SIX different isolates ot Pseudomonas aeuruginosa ( p.
aeruginosa) were collected ,sixteen clinical isolates ( 62% of total group ) were isolated from different samples ( Urine و blood , sputum , wound , ear , eye and throat swabs ) from Medical city hospital in Baghdad, and ten environmental isolates ( 38% of total group ) were isolates from different places of soil and water in the same city .
All isolates were diagnosed by bacteriological and biochemical tests .
ح crude DNA was extracted from all clinical and e^ronmental isolates .
BOX-PCR was achieved using BOX1AR primer .
The BOX-PCR products were analyzed by horizontal agarose gel electrophoresis .
The dendrogram for all isolates was draw by using band-based Jaccard and Dice coefficients where the fingerprints were generated by banding patterns using un weighted pair-group method with arithmetic average ( UPGMA ) , in order to study the relatedness among the clinical and environmental isolates .
The results were showed that the crude DNA was suitable to BOX-PCR , and presence of the high similarity in banding patterns of clinical and environmental isolates itseh^es , which was about 45%-99% and 61%-100% in Jaccard and Dice coefficients respectively .
The si^larity between these two groups was about 40% and 55% in Jaccard and Dice coefficients respectively .
Although , the marked relatedness among all clinical and environmental isolates , this study was concluded presence of genetic variation between the clinical and e^ronmental isolates of this opportunistic bacterium , by using BOX-PCR technique and BOX1AR primer .
American Psychological Association (APA)
Nasir, Ibtesam Hammud. 2013. Use box -pcr to study genetic relatedness of some pseuaomonaus aeruginosa isolates from different clinieal and environmental sourees In Baghdad. Journal of the College of Basic Education،Vol. 19, no. 80, pp.723-732.
https://search.emarefa.net/detail/BIM-705869
Modern Language Association (MLA)
Nasir, Ibtesam Hammud. Use box -pcr to study genetic relatedness of some pseuaomonaus aeruginosa isolates from different clinieal and environmental sourees In Baghdad. Journal of the College of Basic Education Vol. 19, no. 80 (2013), pp.723-732.
https://search.emarefa.net/detail/BIM-705869
American Medical Association (AMA)
Nasir, Ibtesam Hammud. Use box -pcr to study genetic relatedness of some pseuaomonaus aeruginosa isolates from different clinieal and environmental sourees In Baghdad. Journal of the College of Basic Education. 2013. Vol. 19, no. 80, pp.723-732.
https://search.emarefa.net/detail/BIM-705869
Data Type
Journal Articles
Language
English
Record ID
BIM-705869