Use box -pcr to study genetic relatedness of some pseuaomonaus aeruginosa isolates from different clinieal and environmental sourees In Baghdad

Abstract EN

totally twenty SIX different isolates ot Pseudomonas aeuruginosa ( p.

aeruginosa) were collected ,sixteen clinical isolates ( 62% of total group ) were isolated from different samples ( Urine و blood , sputum , wound , ear , eye and throat swabs ) from Medical city hospital in Baghdad, and ten environmental isolates ( 38% of total group ) were isolates from different places of soil and water in the same city .

All isolates were diagnosed by bacteriological and biochemical tests .

ح crude DNA was extracted from all clinical and e^ronmental isolates .

BOX-PCR was achieved using BOX1AR primer .

The BOX-PCR products were analyzed by horizontal agarose gel electrophoresis .

The dendrogram for all isolates was draw by using band-based Jaccard and Dice coefficients where the fingerprints were generated by banding patterns using un weighted pair-group method with arithmetic average ( UPGMA ) , in order to study the relatedness among the clinical and environmental isolates .

The results were showed that the crude DNA was suitable to BOX-PCR , and presence of the high similarity in banding patterns of clinical and environmental isolates itseh^es , which was about 45%-99% and 61%-100% in Jaccard and Dice coefficients respectively .

The si^larity between these two groups was about 40% and 55% in Jaccard and Dice coefficients respectively .

Although , the marked relatedness among all clinical and environmental isolates , this study was concluded presence of genetic variation between the clinical and e^ronmental isolates of this opportunistic bacterium , by using BOX-PCR technique and BOX1AR primer .