تشخيص جزيئي لأنزيم ال sialidase المنتج من قبل بكتريا Gardnerella vaginalis المسببة لخمج المهبل البكتيري

Abstract EN

Include study of 100 swab of vaginal secretion from pregnant and non pregnant women, abortifacient or not abortifacien about( 16-48)years old whom administered to the AL-Zahraa teaching hospital and outpatient in AL-Najaf AL-Ashraf city has been collected during the period from Octobrt 2012 to March 2013.According to Amsel test the results showed highly distribution of BVamong pregnant and non-pergnant as well as abortifacient for one or more than one .The percent of BV infection among pregnant women ,abortifacient more than one about (16-26)years old was higher (%43) than its among non pergnant women ,non abortifacient about( 37-48) years old which was(%11).The results of primary isolation and laboratory diaginosis as well as some biochemical test improved that only 10 isolates were belong to G.vaginalis ,while the results of amplification of 16S rRNA for genetic diagnosis of G.vaginalis by polymerase chain reaction( PCR) showed that 25 isolates were belong to G.vaginalis in vaginal secretion The presence of IS gene encoding sialidase was detected in all vaginal secretion of women suffering from BV by polymerase chain reaction (PCR).The results revealed the presence of SI gene in 24 vaginal secretion samples and only 24 isolates of G.vaginalis had this gene .The study aimed to shed light on bacteria G.

vaginalis as one of the most important causes of bacterial infection of the vagina when women and Doranzim the (sialidase) to increase the ferocity of these bacteria