Oral protein delivery using polyelectrolyte eudragit e-carrageenan complexes and chitosan fatty acid salts
Other Title(s)
التوصيل الفموي للبروتينات باستخدام معقدات الكارجينين اليدروجيت و املاح الاحماض الدهنية للكيتوسان
Dissertant
Thesis advisor
Comitee Members
al-Karad, Jamal
al-Sabawi, Dina
University
Isra University
Faculty
Faculty of Pharmacy
University Country
Jordan
Degree
Master
Degree Date
2015
English Abstract
Encapsulation of proteins in polymeric matrices can offer protection from enzymatic degradation upon oral administration, and thus protein bioavailability can be improved.
Interpolyelectrolyte complexation is used to modify the performance of the polymers in terms of solubility, permeability and swelling.
In this study, bovine serum albumin (BSA), as a model protein, was loaded into microparticles of carrageenan-Eudragit E interpolyelectrolyte complex and chitosan-laurate a hydrophobic salt of chitosan.
The microparticles were characterized for drug encapsulation efficiency and particle size.
The drug release from the microparticles was studied in simulated gastric fluid (SGF) and simulated intestinal fluid (SIF).
Digestibility of the protein as solution and as dispersion of the protein loaded microparticles was examined using a newly developed method that can separate the digestion products from the intact protein.
The microparticles were shown to have acceptable particle size and satisfactory drug loading efficiency.
The protein release from EE-CG microparticles was slower than that from chitosan-laurate and was characterized by being biphasic with burst release followed by slow BSA release.
In comparison to BSA as aqueous solution, the encapsulated protein in the EE-CG microparticles showed a slower rate of enzymatic degradation by pepsin and pancreatin.
However, an enhancement of BSA digestion occurred when it was loaded in chitosan-laurate microparticles, which was explained in terms of enzyme immobilization onto the microparticle matrix.
Main Subjects
Pharmacy, Health & Medical Sciences
No. of Pages
55
Table of Contents
Table of contents.
Abstract.
Abstract in Arabic.
Chapter One : Introduction.
Chapter Two : Methodology.
Chapter Three : Results and discussion.
Chapter Four : Conclusions.
Chapter Five : References.
American Psychological Association (APA)
al-Bdarin, Manal Sidqi Ahmad. (2015). Oral protein delivery using polyelectrolyte eudragit e-carrageenan complexes and chitosan fatty acid salts. (Master's theses Theses and Dissertations Master). Isra University, Jordan
https://search.emarefa.net/detail/BIM-707683
Modern Language Association (MLA)
al-Bdarin, Manal Sidqi Ahmad. Oral protein delivery using polyelectrolyte eudragit e-carrageenan complexes and chitosan fatty acid salts. (Master's theses Theses and Dissertations Master). Isra University. (2015).
https://search.emarefa.net/detail/BIM-707683
American Medical Association (AMA)
al-Bdarin, Manal Sidqi Ahmad. (2015). Oral protein delivery using polyelectrolyte eudragit e-carrageenan complexes and chitosan fatty acid salts. (Master's theses Theses and Dissertations Master). Isra University, Jordan
https://search.emarefa.net/detail/BIM-707683
Language
English
Data Type
Arab Theses
Record ID
BIM-707683
