CTLA-4 gene polymorphisms in women with idiopathic recurrent pregnancy loss

Other Title(s)

التعدد الشكلي لجين مستضدات الخلايا الليمفاوية التائية السامة-4 عند النساء اللاتي تعاني من الإجهاض المتكرر مجهول السبب

Dissertant

Hillis, Iman N.

Thesis advisor

Sharif, Fadil A.

University

Islamic University

Faculty

Faculty of Science

Department

Department of Biological Science

University Country

Palestine (Gaza Strip)

Degree

Master

Degree Date

2016

English Abstract

Background: Recurrent pregnancy loss (RPL) is the miscarriage of two or more consecutive pregnancies before 20th gestational week.

We suggest that dysregulated immunetolerance contributes to idiopathic RPL.

Cytotoxic T lymphocyte associated antigen- 4 (CTLA-4) is considered as a negative regulator of T cell activation and its role in maintaining immune-tolerance is well established.

Objective: The present study aimed to investigate the CTLA-4 +49 A/G , -1661 A/G, -318 C/T and -1722 T/C single nucleotide polymorphisms (SNPs) and predisposition to RPL in Gaza Strip - Palestine.

Methods: This case-control study was performed on DNA samples from 200 women with a history of two or more pregnancy losses (case group) and 200 control women with at least two live births and without any previous history of RPL.

PCR-based restriction fragment length polymorphism (RFLP-PCR) method was used for genotyping CTLA- 4 polymorphisms.

Results: Our study results revealed that there is no significant association between the allele/genotype frequencies of the investigated CTLA-4 SNPs and RPL.

This relation remained true under dominant, co-dominant and recessive models.

The A\G genotype of -1661 A\G polymorphism was higher in patient (45%) as compared to controls (39.5%) but without statistical significance.

The minor allele frequencies (MAFs) of the CTLA-4 gene polymorphisms in the control group were as follows: +49A\G: 0.22, 318 C\T: 0.11, -1661 A\G: 0.26 and -1722T\C: 0.08.

Conclusion: The study showed that there is no significant association between the four investigated CTLA-4 polymorphisms and the risk of RPL in the study population.

Testing other CTLA-4 gene polymorphisms and the level of CTLA-4 expression in RPL patients is recommended.

Key words: Recurrent pregnancy loss, CTLA-4, Gene Polymorphism, PCR-RFLP

Main Subjects

Biology

No. of Pages

Table of contents.

Abstract.

Abstract in Arabic.

Chapter One : Introduction.

Chapter Two : Literature review.

Chapter Three : Methodology.

Chapter Four : Results.

Chapter Five : Discussion.

Chapter Six : Conclusion and recommendations.

References.

American Psychological Association (APA)

Hillis, Iman N.. (2016). CTLA-4 gene polymorphisms in women with idiopathic recurrent pregnancy loss. Islamic University, Palestine (Gaza Strip)
https://search.emarefa.net/detail/BIM-708576

Modern Language Association (MLA)

Hillis, Iman N.. CTLA-4 gene polymorphisms in women with idiopathic recurrent pregnancy loss. Islamic University. (2016).
https://search.emarefa.net/detail/BIM-708576

American Medical Association (AMA)