Development of in House-Enzyme Linked Immunosorbant Assay (ELISA)‎ for sero-diagnosis of Salmonellae infection in patients from Damascus and Daraa governorates

Abstract EN

Typhoid fever induced by Salmonella represents a serious problems, in many regions of the world, including Syria.

Routine diagnosis of suspected typhoid cases is based on detecting agglutinating antibodies in patients sera.

These methods are controversial due to lack of sensitivity and specificity.

New methods like Enzyme linked Immunosorbant Assays (ELISA) for sero-diagnosis of Salmonellae infection have been developed.

Here we describe development of a simple in house-ELISA format for detecting and epidemiological surveillance of typhoid suspected patient.