Detection of MDR gene (IFITM3)‎ and P-glycoprotein expression in patients with Hodgkin’s lymphoma in al-Ramadi Teaching Hospital

Abstract EN

The present study aimed to shed light on the follow up of Hodgkin's lymphoma patients at initial diagnosis and after treatment, to assess the response and early relapse after chemotherapy through evaluating the gene expression level of one of the major multidrug resistance genes which is the multidrug resistance 1 (MDR1),and investigate the presence of P-glycoprotein by immunohistochemistry assay.

The study was conducted on fifty Hodgkin's lymphoma patients during the period extended from January 2015 to November 2015.

The patients included 41(82%) males and 9 (18%) females ,with ages ranged from 12 to 81 years.

The study also included ten persons (7 males and 3 females ) as healthy control group .

All patients were followed up by doing some laboratory test such as complete blood count before and after chemotherapy, and the tumor markers which indicate the response degree of patients to chemotherapy DNA was extracted from paraffin embedded tissue, which were collected from AL-Ramadi teaching Hospital / Histopathology unit, in order to detect the Interferon-induced trans membrane protein IFITM3 (MDR) gene using PCR technique .The results showed in the healthy individuals, there were no mutation presence in the MDR gene in patients and control.

Results of immunohistochemistry study showed that the presence of (MDR1) P glycoprotein had played a great role in regulation of the cancer cells growth.

Depending on the intensity of coloration, and percent of cancer cells stained , the results indicated the production of P-glycoprotein in 15 (30%) case as 1+, 6 (12%) case as 2+ and 10 (20%) case as 3+, while other cases 19 (38%) were negative.