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Purification and characterization of nattokinase produced by local isolate of bacillus Sp. B24
Joint Authors
Hammud, Sumayyah Ali
Aziz, Ghazi Munim
Source
Iraqi Journal of Biotechnology
Issue
Vol. 15, Issue 2 (31 Dec. 2016), pp.93-108, 16 p.
Publisher
University of Baghdad Genetic Engineering and Biotechnology for Postgraduate Studies
Publication Date
2016-12-31
Country of Publication
Iraq
No. of Pages
16
Main Subjects
Topics
Abstract EN
The results presented in this study were based on purifying and characterizing the nattokinase produced from local isolate of Bacillus sp.
B24.
Nattokinase was purified by two steps including the precipitation by ammonium sulfate at 50% saturation and ion exchange chromatography using DEAE-cellulose.
The final purification folds were 16.46 time with an enzyme yield of 30%.
The enzyme was stable in temperatures range 25-45°C and pH values of 8.0–9.5, with maximal activity was defined at 60°C and pH value of 8.0, whereas was retained 94% of its activity at 60˚C for 20 min.
The presence of KCl, CaCl2, MnCl2, NaCl and MgCl2 was observed to enhance enzyme activity to levels above their original activity, whereas CoCl2, HgCl2 and FeCl3 decreased the enzyme activity.
Furthermore, EDTA and PMSF strongly inhibited the enzyme activity suggesting that it is a metalloprotease and serine protease enzyme.
The enzyme molecular weight as determined by gel filtration using Sephadex G-150 was found to be 63 kDa.
Fibrinolytic activity of the partial purified nattokinase was studied in vitro conditions and it was found that the enzyme has degradation effect
American Psychological Association (APA)
Hammud, Sumayyah Ali& Aziz, Ghazi Munim. 2016. Purification and characterization of nattokinase produced by local isolate of bacillus Sp. B24. Iraqi Journal of Biotechnology،Vol. 15, no. 2, pp.93-108.
https://search.emarefa.net/detail/BIM-709494
Modern Language Association (MLA)
Hammud, Sumayyah Ali& Aziz, Ghazi Munim. Purification and characterization of nattokinase produced by local isolate of bacillus Sp. B24. Iraqi Journal of Biotechnology Vol. 15, no. 2 (2016), pp.93-108.
https://search.emarefa.net/detail/BIM-709494
American Medical Association (AMA)
Hammud, Sumayyah Ali& Aziz, Ghazi Munim. Purification and characterization of nattokinase produced by local isolate of bacillus Sp. B24. Iraqi Journal of Biotechnology. 2016. Vol. 15, no. 2, pp.93-108.
https://search.emarefa.net/detail/BIM-709494
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 107-108
Record ID
BIM-709494