Genotyping of Klebsiella spp. isolated from different clinical sources
Other Title(s)
التنميط الجيني لبكتريا Klebsiella spp المعزولة من مصادر سريرية مختلفة
Joint Authors
al-Rabii, Dalal Salih
Hamzah, Hibah Muhammad
Abd al-Rahman, Thana Rashid
Source
Issue
Vol. 57, Issue 3B (30 Sep. 2016), pp.1937-1951, 15 p.
Publisher
University of Baghdad College of Science
Publication Date
2016-09-30
Country of Publication
Iraq
No. of Pages
15
Main Subjects
Topics
Abstract EN
A total of 172 clinical were obtained over 6 months.
Klebsiella spp.
was detected in 58 (33.7%) samples with a high percentage 29 (50%) in urine in female and low percentage 1(1.7%) in pus and burn swabs in male, and the vaginal swab was 1(1.7%).
The female to male ratio was 3.1:1.
PCR detection showed that 51(87.93%) out of 58 produce 108 bp.
product with rpoB specific primer that represented K.
pneumonia.
Whereas 7(12.07%) showed PCR product with 343 bp by K.
oxytoca specific primer (peh X), furthermore, the sequences of two selected isolates showed that the species related to K.
oxytoca strain CAV1335, and to K.
oxytoca strain CAV1374.
Five selected isolates were re-tested by the gyr A primer, all were showed specific band product with 441bp.
Sequencing blast analysis for these isolates showed that one was related to K.
pneumoniae subsp.
pneumoniae strain RJF999, two isolates related to K.
pneumoniae strain 17265 GyrA, one related to K.
oxytoca strain 7102 GyrA and one related to K.
pneumonia isolate 103 GyrA gene.
Phylogenetic tree analysis showed the relation of 3 K.
pneumoniae isolates to USA and UK strains and one with the Asian strains, and 2 K.
oxytoca isolates have a relation within the Iranian strains and one has a genetic variation.
American Psychological Association (APA)
al-Rabii, Dalal Salih& Abd al-Rahman, Thana Rashid& Hamzah, Hibah Muhammad. 2016. Genotyping of Klebsiella spp. isolated from different clinical sources. Iraqi Journal of Science،Vol. 57, no. 3B, pp.1937-1951.
https://search.emarefa.net/detail/BIM-709551
Modern Language Association (MLA)
al-Rabii, Dalal Salih…[et al.]. Genotyping of Klebsiella spp. isolated from different clinical sources. Iraqi Journal of Science Vol. 57, no. 3B (2016), pp.1937-1951.
https://search.emarefa.net/detail/BIM-709551
American Medical Association (AMA)
al-Rabii, Dalal Salih& Abd al-Rahman, Thana Rashid& Hamzah, Hibah Muhammad. Genotyping of Klebsiella spp. isolated from different clinical sources. Iraqi Journal of Science. 2016. Vol. 57, no. 3B, pp.1937-1951.
https://search.emarefa.net/detail/BIM-709551
Data Type
Journal Articles
Language
English
Notes
Text in English ; abstracts in English and Arabic.
Record ID
BIM-709551