The alteration of mRNA expression of SOD and GPX genes, and proteins in tomato (Lycopersicon esculentum mill) under stress of NaCl and or ZnO nanoparticles
Joint Authors
al-Harbi, Hisham F.
al-Dhibani, Amal Y.
Mutawalli, Ihab M. R.
Fuller, Michael P.
Source
Saudi Journal of Biological Sciences
Issue
Vol. 23, Issue 6 (30 Nov. 2016), pp.773-781, 9 p.
Publisher
Publication Date
2016-11-30
Country of Publication
Saudi Arabia
No. of Pages
9
Main Subjects
Pharmacy, Health & Medical Sciences
Medicine
Abstract EN
Five cultivars of tomato having different levels of salt stress tolerance were exposed to different treatments of NaCl (0, 3 and 6 g L _1) and ZnO-NPs (0, 15 and 30 mg L _1).
Treatments with NaCl at both 3 and 6 g L _1 suppressed the mRNA levels of superoxide dismutase (SOD) and glutathione peroxidase (GPX) genes in all cultivars while plants treated with ZnO-NPs in the presence of NaCl, showed increments in the mRNA expression levels.
This indicated that ZnO-NPs had a positive response on plant metabolism under salt stress.
Superior expression levels of mRNA were observed in the salt tolerant cultivars, Sandpoint and Edkawy while the lowest level was detected in the salt sensitive cultivar, Anna Aasa.
SDS–PAGE showed clear differences in patterns of protein expression among the cultivars.
A negative protein marker for salt sensitivity and ZnO-NPs was detected in cv.
Anna Aasa at a molecular weight of 19.162 kDa, while the tolerant cultivar Edkawy had two positive markers at molecular weights of 74.991 and 79.735 kDa.
_ 2016 The Authors.
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This is an open access article under theCCBY-NC-NDlicense (http://creativecommons.org/licenses/by-nc-nd/4.0/).
American Psychological Association (APA)
al-Harbi, Hisham F.& Mutawalli, Ihab M. R.& Fuller, Michael P.& al-Dhibani, Amal Y.. 2016. The alteration of mRNA expression of SOD and GPX genes, and proteins in tomato (Lycopersicon esculentum mill) under stress of NaCl and or ZnO nanoparticles. Saudi Journal of Biological Sciences،Vol. 23, no. 6, pp.773-781.
https://search.emarefa.net/detail/BIM-717181
Modern Language Association (MLA)
al-Harbi, Hisham F.…[et al.]. The alteration of mRNA expression of SOD and GPX genes, and proteins in tomato (Lycopersicon esculentum mill) under stress of NaCl and or ZnO nanoparticles. Saudi Journal of Biological Sciences Vol. 23, no. 6 (Nov. 2016), pp.773-781.
https://search.emarefa.net/detail/BIM-717181
American Medical Association (AMA)
al-Harbi, Hisham F.& Mutawalli, Ihab M. R.& Fuller, Michael P.& al-Dhibani, Amal Y.. The alteration of mRNA expression of SOD and GPX genes, and proteins in tomato (Lycopersicon esculentum mill) under stress of NaCl and or ZnO nanoparticles. Saudi Journal of Biological Sciences. 2016. Vol. 23, no. 6, pp.773-781.
https://search.emarefa.net/detail/BIM-717181
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 780-781
Record ID
BIM-717181