Spectrophotometric method for pregabalin determination : an experimental design approach for method development
Joint Authors
Patil, Dipak D.
Wani, Yogita B.
Source
Arab Journal of Basic and Applied Sciences
Issue
Vol. 21, Issue 0 (31 Oct. 2016), pp.31-37, 7 p.
Publisher
University of Bahrain College of Science
Publication Date
2016-10-31
Country of Publication
Bahrain
No. of Pages
7
Main Subjects
Topics
Abstract EN
A simple, sensitive and reproducible spectrophotometric method is developed for deter-mining the pregabalin (PGB) content in bulk and in capsule dosage form using an experimental design approach.
The proposed method is based on the condensation reaction of PGB (primary amine) with p-dimethylaminobenzaldehyde (PDAB) in an acidic medium to form a PGB–PDAB complex.
The PGB–PDAB complex shows maximum absorption at 395.80 nm.
The proposed method is validated according to the ICH Q2 (R1) guidelines for validation of analytical methods.
The percentage purity of PGB in capsule dosage form as determined using the proposed method is 100.05 ± 1.48 whereas the corresponding value by the official method (Indian Pharmacopoeia 2010) is 100.46 ± 0.41.
The t-value and F-value are calculated for statistical comparison and are found to be 0.60 and 0.08, respectively.
The proposed method may recommend for routine quality control analysis of PGB in its pharmaceutical dosage form.
American Psychological Association (APA)
Patil, Dipak D.& Patil, Mukesh S.& Wani, Yogita B.. 2016. Spectrophotometric method for pregabalin determination : an experimental design approach for method development. Arab Journal of Basic and Applied Sciences،Vol. 21, no. 0, pp.31-37.
https://search.emarefa.net/detail/BIM-717716
Modern Language Association (MLA)
Patil, Mukesh S.…[et al.]. Spectrophotometric method for pregabalin determination : an experimental design approach for method development. Arab Journal of Basic and Applied Sciences Vol. 21 (Oct. 2016), pp.31-37.
https://search.emarefa.net/detail/BIM-717716
American Medical Association (AMA)
Patil, Dipak D.& Patil, Mukesh S.& Wani, Yogita B.. Spectrophotometric method for pregabalin determination : an experimental design approach for method development. Arab Journal of Basic and Applied Sciences. 2016. Vol. 21, no. 0, pp.31-37.
https://search.emarefa.net/detail/BIM-717716
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 37
Record ID
BIM-717716