Comparative study between liquid state fermentation and solid state fermentation for production of endo-1, 4-beta-xylanase by Bacillus licheniformis

Abstract EN

Bacillus licheniformis isolated from soil was determined as the most efficient xylanase producer, while the isolates from rice and wheat samples did not produce this enzyme.

Maximum xylanase production could be achieved in Liquid State Fermentation (LSF) with inoculum size of 1.5 ml and after an incubation period 48 hours, at 40 ºC and pH 7.

Birchwood xylan and yeast extract were found to be the best carbon and nitrogen sources respectively, for producing high level of xylanase.

Solid state fermentation gave higher xylanase production in comparative with liquid state fermentation.

Different fermentation conditions were standardized for xylanase production in Solid State Fermentation (SSF), the optimum being 72 hours growth at pH 6.0, cultivation temperature 50 ° C and substrate to moisture ration of 1:2 (w/v).

Among different lignocelluloses substrates a mixture of wheat bran and corn bran was found to be the best substrate for xylanase production.

The inoculum size of 2 ml resulted in maximum production of xylanase.