العلاقة بين شذوذات النطاف الصبغية و النطاف غير السوية مورفولوجيا لدى عينة من المرضى السوريين المصابين بقلة النطاف الوخيمة و المعتدلة

Abstract EN

Intracytoplasmic sperm injection (ICSI) technique is considered to be one of the most important methods which are used for infertility cases of men, especially when the infertility is correlated with abnormal semen parameters.

Many studies show an increased frequency of chromosome abnormalities in the sperms of men with certain cases of infertility and undergoing ICSI.

Trying to find weather the presence of a defined morphological abnormality of sperm can be related to a defined numerical chromosomal abnormality in infertile men with low sperm concentration (severe and mild oligozoospermia).

23 infertile men with oligozoospermia were studied and 5 control cases.

Semen samples were collected for semen evaluation, and the numerical chromosomal abnormalities of the chromosomes 13, 18, 21, and sex chromosomes using the fluorescence in situ hybridization.

These results showed the presence of a reverse correlation between the concentration of sperms and the aneuploidy rates of chromosomes 13, 21, and sex chromosomes.

And some incorporeal correlations were found between the percentages of spermatozoa with specific malformations and particular chromosomal abnormalities, we found that the elongated heads correlate with the 13 Disomy, and the big head was related to the XY Disomy, the irregular middle piece defect shows relation with the 21 Disomy, and at last the absent tail correlated with the total aneuploidy cases of sex chromosomes (XY).

In conclusion, our results suggest avoiding choosing sperms with such morphological abnormalities while using ICSI technique, especially in the persons whom they have a very low total count of sperm (the samples of this study) where it’s difficult to find morphologically normal sperms.

And they insure the importance of using FISH technique on sperm, because it gives the percentage of chromosomal abnormalities in sperm, and shows the expiry of using ICSI technique for infertility patients, and in case this was not possible, advice the couples who suffer from infertility caused by severe oligozoospermia and undergoing ICSI to do the PGD test which explore the aneuploidy of chromosomes in the fetus before transfer it into the uterus.