The influence of some amino acids, vitamins and anti-inflammatory drugs on activity of chondroitinase produced by Proteus vulgaris caused urinary tract infection
Other Title(s)
تأثير بعض الأحماض الأمينية و الفيتامينات و بعض العقاقير المسكنة للألام على فعالية أنزيم ال chondroitinas المنتج من بكتريا proteus vulgaris المسببة لالتهاب المسالك البولية
Joint Authors
al-Imam, Mukhtar Jawad Kazim
al-Rabii, Baha Abd Allah Laftah
Source
Issue
Vol. 57, Issue 4A (31 Dec. 2016), pp.2412-2421, 10 p.
Publisher
University of Baghdad College of Science
Publication Date
2016-12-31
Country of Publication
Iraq
No. of Pages
10
Main Subjects
Topics
Abstract EN
Two hundred and ten specimens include urine, blood and ear swab were collected from different hospitals in Baghdad city; 85 (40%) isolates were diagnosed as Proteus spp.
with (82%), (11.7%) and (5.8%) represented in urine, blood and ear swab specimens respectively.
PCR technique was shown 30 (35.3%) isolates were positive for specific Urease C gene that used in rapid detection of Proteus vulgaris.
The ability for chondritinase production was checked invetro and invevo, 24 (80%) isolates of P.
vulgaris were showed ablity to chondritinase production and the isolate (p17) has higher enzyme activity value to (175.2U/ml).
The Chondroitinase was purified by three short steps only included precipitate with 60% saturated of ammonium sulfate, dialysis and then filtrated by Sephadex G-150.
The enzymatic activity was peaked to (270U/ml) and specific activity (692.3U/mg).
The enzymatic activity for purified enzyme was increased significantly (P<0.01) to (563.7U/ml) after treatment with 1M of tyrosine but aspartic acid and glutamic acid were show inhibitory effect on enzyme activity decreased (P<0.01) to (130.U/ml) and (147.3 U/ml) respectively.
The enzymatic activity was also increased significantly (P<0.01) reached to (633.6U/ml) and to(610.3U/ml) when treated with 50% of vitamin k and piroxicam respectively.
This study was aimed to investigate effect of amino acids, vitamins and some nonsteroidal anti-inflammatory drugs (NSAIDs) with different concentrations on enzyme activity
American Psychological Association (APA)
al-Imam, Mukhtar Jawad Kazim& al-Rabii, Baha Abd Allah Laftah. 2016. The influence of some amino acids, vitamins and anti-inflammatory drugs on activity of chondroitinase produced by Proteus vulgaris caused urinary tract infection. Iraqi Journal of Science،Vol. 57, no. 4A, pp.2412-2421.
https://search.emarefa.net/detail/BIM-722688
Modern Language Association (MLA)
al-Imam, Mukhtar Jawad Kazim& al-Rabii, Baha Abd Allah Laftah. The influence of some amino acids, vitamins and anti-inflammatory drugs on activity of chondroitinase produced by Proteus vulgaris caused urinary tract infection. Iraqi Journal of Science Vol. 57, no. 4A (2016), pp.2412-2421.
https://search.emarefa.net/detail/BIM-722688
American Medical Association (AMA)
al-Imam, Mukhtar Jawad Kazim& al-Rabii, Baha Abd Allah Laftah. The influence of some amino acids, vitamins and anti-inflammatory drugs on activity of chondroitinase produced by Proteus vulgaris caused urinary tract infection. Iraqi Journal of Science. 2016. Vol. 57, no. 4A, pp.2412-2421.
https://search.emarefa.net/detail/BIM-722688
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 2420-2421
Record ID
BIM-722688