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Pathogenesis of campylobacter jejuni infection with emphasis on ultra structural changes
Other Title(s)
استخدام المجهر الإلكتروني لدراسة أمراضية جراثيم C. jejuni
Joint Authors
al-Dulaymi, Muna S.
al-Hadithi, Raji H.
Source
Issue
Vol. 2, Issue 1 (31 Dec. 2008), pp.17-30, 14 p.
Publisher
University of Duhok College of Medicine
Publication Date
2008-12-31
Country of Publication
Iraq
No. of Pages
14
Main Subjects
Pharmacy, Health & Medical Sciences
Abstract EN
Aim Studying comprehensively the pathogenesis of local isolate of C.jejuni in our country with their details by using ultrastructural studies depending on suitable protocol and animal model.
Methods Newly local isolate of C.jejuni (CJM6) which isolated from children was used to study the pathogenesis of such bacteria after oral administration of 1.7x10 viable cell /ml for gnotobiotic mice which were as animal model .
Results Under scanning electron microscopy (SEM), the earliest colonization with huge numbers of C.jejuni appear at 24 hours post inoculation (P.I), and early adherence at 48 hours P.I with normal mucosal appearance.
The mucosal and edema and loss of microvilli in some areas of epithelial surface were observed 3 and 4 days after inoculation , due to early penetration of C.jejuni.
In 5 days after inoculation, the mucosa reveled irregular opening of cecal crypts with reduction of goblet cells numbers as well as destroyed cecal mucosa.
While 6 and 7 days P.I revealed patchy erosion and necrosis with persistence adherence and colonization.
Under transmission electron microscopy (TEM) the colonization was seen within the mucous environment of cecal epithelium, with normal appearance of cellular details.
3 and 4 days P.I some cases showed mixed healthy cells and other showed abnormalities of microvilli, as well as presence of free invasive C.jejuni, within epithelial cytoplasm, while deeper crypts were seen to be heavily colonized.
Degenerative changes included partial loss of surface mucosal microvilli with numerous invasive C.jejuni mucosal goblet cells, while others seen within membrane vacuole in cytoplasmic epithelial cells 5 days P.I.
At later stage of infection degenerative changes of microvilli ranged from elongation, fusion swelling, budding to abnormal shortening and microvillous-cytoplasmic extrusion toward C.jejuni within cecal lumen as well as exfoliated microvilli and apical cytoplasm into lumen.
Conclusion Gnotobiotic mice improved to be suitable model for studying pathogenesis by producing transient bacteremia, diarrhea and intestinal lesions resembling that which may occur in human.
EM (SEM & TEM) is an important in increasing our understanding of disease pathogenesis which include colonization, adherence, penetration, multiplication and invasion as well as producing several pathological changes.
American Psychological Association (APA)
al-Dulaymi, Muna S.& al-Hadithi, Raji H.. 2008. Pathogenesis of campylobacter jejuni infection with emphasis on ultra structural changes. Duhok Medical Journal،Vol. 2, no. 1, pp.17-30.
https://search.emarefa.net/detail/BIM-722840
Modern Language Association (MLA)
al-Dulaymi, Muna S.& al-Hadithi, Raji H.. Pathogenesis of campylobacter jejuni infection with emphasis on ultra structural changes. Duhok Medical Journal Vol. 2, no. 1 (2008), pp.17-30.
https://search.emarefa.net/detail/BIM-722840
American Medical Association (AMA)
al-Dulaymi, Muna S.& al-Hadithi, Raji H.. Pathogenesis of campylobacter jejuni infection with emphasis on ultra structural changes. Duhok Medical Journal. 2008. Vol. 2, no. 1, pp.17-30.
https://search.emarefa.net/detail/BIM-722840
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 27-28
Record ID
BIM-722840