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Biological and analytical variation of serum lipid profile
Other Title(s)
التباين البايولوجي و التحليلى لشحوم مصل الدم
Joint Authors
Mawla Abd, Wad Allah S.
Salih, Saba K.
Source
Issue
Vol. 2, Issue 1 (31 Dec. 2008), pp.41-53, 13 p.
Publisher
University of Duhok College of Medicine
Publication Date
2008-12-31
Country of Publication
Iraq
No. of Pages
13
Main Subjects
Pharmacy, Health & Medical Sciences
Abstract EN
Background Dyslipidaemia is a major risk factor for coronary heart disease which can be assessed by measuring serum lipid profile.
Biological variation has an important effect on the interpretation of all laboratory investigations, including lipid profile.
Objectives To define the biological and analytical components of variation for the different parameters of serum lipid profile.
Methods The present study was conducted in Mosul City in northern Iraq, from 1st February to 30th April 2004.
Fasting venous blood was collected from each of 10 apparently healthy volunteers (6 men and 4 women, aged 22-40 years), at 8-10 am following an overnight fast, at intervals of one week for 10 weeks.
Sera were separated and stored frozen, in duplicate.
Measurement and calculation of the different components of serum lipid profile were made including: triglycerides (TG), total cholesterol, HDL-C, LDL-C and ratios of total cholesterol: HDL-C, LDL-C: HDL-C and TG: HDL-C.
Results The intra-individual (CVI) and inter-individual (CVG) variation were 21% and 37% for TG, 7.5% and 16.7% for total cholesterol, 11.2% and 24.5% for HDL-C, 13.7% and 28.3% for LDL-C, 13.1% and 25.4% for total cholesterol: HDL-C, 25.9% and 34.7% for LDL-C: HDL-C, and 27.2% and 40.7% for TG: HDL-C respectively.
The indices of individuality, as reflected by CVI/CVG, for these parameters were all <1.0 (0.57 for TG, 0.45 for total cholesterol, 0.46 for HDL-C, 0.48 for LDL-C, 0.52 for total cholesterol: HDL-C, 0.95 for LDL-C: HDL-C and 0.85 for TG: HDL-C).
The analytical goals for imprecision, as reflected by analytical variation (CVA), was 6.3% for TG, 4.0% for total cholesterol, 5.2% for HDL-C, 7.8% for LDL-C, 5.8% for total cholesterol: HDL-C, 5.7% for LDL-C: HDL-C and 5.9% for TG: HDL-C.
The critical difference calculated as 2.77(CVA2+ CVI2)1/2 was 60.7% for TG, 23.5% for total cholesterol, 34.2% for HDL-C, 43.6% for LDL-C, 39.7% for total cholesterol: HDL-C, 73.3% for LDL-C: HDL-C and 97.5% for TG: HDL-C.
Conclusion The biological and analytical components of variation showed marked individuality.
This together with the index of individuality supports the limited usefulness of using the conventional population-based reference range for interpretive criteria.
The critical differences also confirm that single determination of lipid profile may have limited value in screening purpose.
American Psychological Association (APA)
Mawla Abd, Wad Allah S.& Salih, Saba K.. 2008. Biological and analytical variation of serum lipid profile. Duhok Medical Journal،Vol. 2, no. 1, pp.41-53.
https://search.emarefa.net/detail/BIM-722842
Modern Language Association (MLA)
Mawla Abd, Wad Allah S.& Salih, Saba K.. Biological and analytical variation of serum lipid profile. Duhok Medical Journal Vol. 2, no. 1 (2008), pp.41-53.
https://search.emarefa.net/detail/BIM-722842
American Medical Association (AMA)
Mawla Abd, Wad Allah S.& Salih, Saba K.. Biological and analytical variation of serum lipid profile. Duhok Medical Journal. 2008. Vol. 2, no. 1, pp.41-53.
https://search.emarefa.net/detail/BIM-722842
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 49-51
Record ID
BIM-722842