Specific identification of Campylobacter Jejuni using a PCR assay based on the hip-o gene

Abstract EN

Background and objectives In recent years, the use of molecular methods based on polymerase chain reaction amplification may provide an alternative to classical methods and are increasingly applied to the detection and confirmation identification of Campylobacter jejuni cultures directly.

The aim of this study was to confirm the identification of phenotypically-identified C.

jejuni isolates by polymerase chain reaction assays using a species-specific primer, hipO.

Methods From 48 previously phenotypically-identified C.

jejuni isolates, 12 isolates were selected according to differences in their sources, biotyping and resistotyping patterns.

These isolates were subjected to species-specific polymerase chain reaction assay using hipO primer.

Results hipO primer produced appropriate and successful results which yielded amplified products with all selected isolates.

Conclusions Using hipO primer makes further confirmation of phenotypically-identified C jejuni by subjection them to species-specific polymerase chain reaction assay with specificity 100%.