The synergistic effects of rutin and urate oxidase on nephrotoxicity in rats

Abstract EN

rutin or/and urate oxidase in gentamicin (GM) induced nephrotoxic rats.

Two experiments were carried out, the first one showed that daily injection of 80 mg GM/kg b.wt ineraperitonealy (I.P) for two weeks induced acute renal failure indicated by significant elevation in serum urea, creatinine, uric acid, potassium, inorganic phosphorus, parathyroid hormone and thiobarbituric acid reactive substance and a significant decline in serum sodium, total & ionized calcium with a pronounce decrease in the content of glutathione and the activities of oxidized glutathione , glutathione peroxidase, superoxide dismutase and catalase when compared with their corresponding values in saline injected rats.

In the second experiment, comparisons were made between GM induced nephrotoxic rat and other nephrotoxic groups received daily additional oral rutin (100 mg/kg diet), urate oxidase injection (10 mg/100 g b.wt.) and their mixture for 15 & 30 days after the incidence of nephrotoxicity.

All the previous parameters were corrected to some extent after the nephrotoxic rats treated with rutin, urate oxidase and their combination.