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Preparation and validation of double antibody radioimmunoassay for thyroglobulin (Tg) using balb C mice as host animals
Joint Authors
Abd al-Ghani, I. Y.
Shafiq, H. M.
al-Mouhti, N. R. A.
Source
Arab Journal of Nuclear Sciences and Applications
Issue
Vol. 47, Issue 2 (30 Apr. 2014), pp.233-240, 8 p.
Publisher
The Egyptian Society of Nuclear Science and Applications
Publication Date
2014-04-30
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Abstract EN
The preparation and development of primary reagents of thyroglobulin (Tg) radioimmunoassay technique with low cost is considered to be the main objective of present study.
The production of polyclonal antibodies of thyroglobulin was undertaken by immunizing three bulb/C mice intraperitonial through primary injection and two booster doses.
The preparation of 125I-Tg radiotracer was carried out using chloramine-T as oxidizing agent.
The preparation of thyroglobulin standards were carried out.
Optimization and validation of the assay were studied out.
The results obtained provide a highly sensitive, specific and accurate RIA system for thyroglobulin based on liquid phase separation.
In conclusion, this assay could be used for diagnosis of thyroid cancer.
American Psychological Association (APA)
Abd al-Ghani, I. Y.& Shafiq, H. M.& al-Mouhti, N. R. A.. 2014. Preparation and validation of double antibody radioimmunoassay for thyroglobulin (Tg) using balb C mice as host animals. Arab Journal of Nuclear Sciences and Applications،Vol. 47, no. 2, pp.233-240.
https://search.emarefa.net/detail/BIM-724178
Modern Language Association (MLA)
Abd al-Ghani, I. Y.…[et al.]. Preparation and validation of double antibody radioimmunoassay for thyroglobulin (Tg) using balb C mice as host animals. Arab Journal of Nuclear Sciences and Applications Vol. 47, no. 2 (Apr. 2014), pp.233-240.
https://search.emarefa.net/detail/BIM-724178
American Medical Association (AMA)
Abd al-Ghani, I. Y.& Shafiq, H. M.& al-Mouhti, N. R. A.. Preparation and validation of double antibody radioimmunoassay for thyroglobulin (Tg) using balb C mice as host animals. Arab Journal of Nuclear Sciences and Applications. 2014. Vol. 47, no. 2, pp.233-240.
https://search.emarefa.net/detail/BIM-724178
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 239-240
Record ID
BIM-724178