Synthesis of 125I-salicyl hydroxamic acid for urinary bladder imaging

Abstract EN

Salcylichydroxamic acid is a salicylate derivative.

Radiolabeling of Salicylhydroxamic acid (SHA) with iodine-125 may have considerable interest for imaging of urinarybladder.

This study is aimed to optimize the radiolabeling yield of Salcylhydroxamic with radioiodine (125-123) using chloreamine-T (CAT) as an oxidizing agent with respect to factors that affect the reaction conditions such as SHA amount, CAT amount, reaction time and pH of the reaction mixture.

In-vitro stability of the radiolabeled complex was checked and it was found to be stable for up to 24 h.

125I-SHA was injected via intravenous administration routes into normal male Sprague–Dawley rats.

Bio-distribution studies have revealed that 125I-SHA was excreted in urine with extent that it could give a clear image for urinary bladder especially if the bladder it tightly closed.

The amount of 125I-Salcyl hydroxamic excreted was increased in case of giving potassium bicarbonate to rat before injection of 125I-SHA.

The result of bio-distribution study of 125I-SHA in experimental animal suggested the possibility of using 123I-SHA to image the urinary bladder.