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Studies on purification and coatation of polyclonal antibody for prolactin solid phase radioimmunoassay in human serum
Joint Authors
Salam, Kh. M.
Shafiq, H. M.
al-Bayyumi, A. S. A.
Source
Arab Journal of Nuclear Sciences and Applications
Issue
Vol. 47, Issue 3 (30 Jun. 2014), pp.139-147, 9 p.
Publisher
The Egyptian Society of Nuclear Science and Applications
Publication Date
2014-06-30
Country of Publication
Egypt
No. of Pages
9
Main Subjects
Abstract EN
The objective of the present study was oriented to produce purified polyclonal antibody to prepare a prolactin solid phase coated tubes radioimmunoassay system.
In the present study, production of polyclonal antibodies was carried out through immunization of three healthy white male mature New Zealand rabbits with a highly purified sheep prolactin antigen.
The obtained anti-sera was purified using an anion exchange reactive group, diethylaminoethyle (DEAE) covalently linked to Sepharose.
The purified polyclonal antibody was used for coating polystyrene tubes.
The preparation of 125I-prolactin tracer was carried out using chloramine-T method.
The preparation of standards was performed using assay buffer to cover the range from 2 to 200 ng/ml.
The optimization and validation tests of the assay were performed to evaluate the validity of the prepared system.
In conclusion, this low cost assay would be used in diagnosis of pituitary dysfunction and diagnosis of infertility in males and females.
American Psychological Association (APA)
al-Bayyumi, A. S. A.& Salam, Kh. M.& Shafiq, H. M.. 2014. Studies on purification and coatation of polyclonal antibody for prolactin solid phase radioimmunoassay in human serum. Arab Journal of Nuclear Sciences and Applications،Vol. 47, no. 3, pp.139-147.
https://search.emarefa.net/detail/BIM-724829
Modern Language Association (MLA)
al-Bayyumi, A. S. A.…[et al.]. Studies on purification and coatation of polyclonal antibody for prolactin solid phase radioimmunoassay in human serum. Arab Journal of Nuclear Sciences and Applications Vol. 47, no. 3 (Jun. 2014), pp.139-147.
https://search.emarefa.net/detail/BIM-724829
American Medical Association (AMA)
al-Bayyumi, A. S. A.& Salam, Kh. M.& Shafiq, H. M.. Studies on purification and coatation of polyclonal antibody for prolactin solid phase radioimmunoassay in human serum. Arab Journal of Nuclear Sciences and Applications. 2014. Vol. 47, no. 3, pp.139-147.
https://search.emarefa.net/detail/BIM-724829
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 146-147
Record ID
BIM-724829