Detection of some pathogenic bacteria in chicken meat sold in poultry shops

Abstract EN

The current study was done to evaluate the microbiological status of some of the retailed chicken meat.

A total of 50 random samples of chicken meat were collected from different poultry shops at Kafrelshiekh Governorate.

The samples were evaluated bacteriologically for the detection of some food poisoning pathogens (Salmonella, E.coli and Staphylococcus aureus).

The obtained results revealed that Salmonella spp.

were isolated with a percentage of 18%, furthermore, the serological identification of the obtained isolates revealed the presence of S.

Typhimurium (6%), S.

Enteritidis (4%), S.

Kentucky (4%), S.

Molade (2%) and S.

Infants (2%).

On the other hand, E.coli was detected in 12% of the examined sample, and the serological identification of the obtained isolates revealed the presence of the following serotypes O78, O103:H2, O1:H7 and O125:H21.

About 20% (10 isolates) of the examined samples were belonging to Staph.

aureus.

Detection of Staph.

aureus enterotoxin by using multiplex PCR indicates that only 4 strains from the 10 isolates were positive for enterotoxin production One isolate produce Sea, Sec; One isolate produce Sea; one isolate Sec; and the last isolate produce Sea, Seb, Sed enterotoxin.

Based on the obtained results, it was concluded that chicken meat poses high risk for public heath, so strict hygienic measures should be taken during slaughtering and processing to prevent cross contamination.