Prevalence, pathogenicity and antibiogram sensitivity of Pseudomonas Aeruginosa isolated from diseased chickens

Abstract EN

Pseudomonas aeruginosa is an opportunistic pathogen which produces several diseases and considered all over the world as one of the most dangerous diseases which affect animals.

A total of thirteen isolates were identified as P.

aeruginosa with percentage of 6.5%, 7.3% from diseased and dead chicken and 4% from one day old chicks.

Polymerase chain reaction (PCR) for detection of oprL gene of P.

aeruginosa was applied and showed positive amplification of 504 pb fragments.

The isolates were found to be resistant for Neomycin, Nalidixic acid and Lincomycin followed by Trimethoprim-sulfamethoxazole, Chloramphenicol then Tetracycline and Doxycyclin but appeared sensitive to Levofloxacin with percentage of 100%, 92.3% to Norfloxacin, 84.6% to Ciprofloxacin, 77% to both Colistin sulphate and Gentamicin but 61.5% to streptomycin.

In experimental infection for all isolated strains via yolk sac in 7 days SPF eggs and via subcutaneous injection in 3 days old chicks which expressed the virulence of these strains by its high mortality rate which reached 100% within 24-72 hrs.