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Quantitative analysis of plasma cell-free DNA and its DNA integrity in patients with metastatic prostate cancer using ALU sequence
Joint Authors
al-Fayyumi, Hanin Muhammad
Swaifi, Karimah
Nawfal, Najwa
Fawzi, Amal
Source
Journal of the Egyptian National Cancer Institute
Issue
Vol. 28, Issue 4 (31 Dec. 2016), pp.235-242, 8 p.
Publisher
Cairo University National Cancer Institute
Publication Date
2016-12-31
Country of Publication
Egypt
No. of Pages
8
Main Subjects
Pharmacy, Health & Medical Sciences
Abstract EN
Background : Prostate cancer (PC) is the most common cancer affecting men, it accounts for 29 % of all male cancer and 11 % of all male cancer related death.
DNA is normally released from an apoptotic source which generates small fragments of cell-free DNA, whereas cancer patients have cell-free circulating DNA that originated from necrosis, autophagy, or mitotic catastrophe, which produce large fragments.
Aim of work : Differentiate the cell free DNA levels (cfDNA) and its integrity in prostate cancer patients and control group composed of benign prostate hyperplasia (BPH) and healthy persons.
Methodology : cf-DNA levels were quantified by real-time PCR amplification in prostate cancer patients (n = 50), (BPH) benign prostate hyperplasia (n = 25) and healthy controls (n = 30) using two sets of ALU gene (product size of 115 bp and 247-bp) and its integrity was calculated as a ratio of qPCR results of 247 bp ALU over 115 bp ALU.
Results : Highly significant levels of cf-DNA and its integrity in PC patients compared to BPH.
Twenty-eight (56 %) patients with prostate cancer had bone metastasis.
ALU115 qpcr is superior to the other markers in discriminating metastatic patients with a sensitivity of 96.4% and a specificity of 86.4% and (AUC= 0.981) Conclusion : ALU115 qpcr could be used as a valuable biomarker helping in identifying high risk patients, indicating early spread of tumor cells as a potential seed for future metastases.
American Psychological Association (APA)
Fawzi, Amal& Swaifi, Karimah& al-Fayyumi, Hanin Muhammad& Nawfal, Najwa. 2016. Quantitative analysis of plasma cell-free DNA and its DNA integrity in patients with metastatic prostate cancer using ALU sequence. Journal of the Egyptian National Cancer Institute،Vol. 28, no. 4, pp.235-242.
https://search.emarefa.net/detail/BIM-726059
Modern Language Association (MLA)
Swaifi, Karimah…[et al.]. Quantitative analysis of plasma cell-free DNA and its DNA integrity in patients with metastatic prostate cancer using ALU sequence. Journal of the Egyptian National Cancer Institute Vol. 28, no. 4 (Dec. 2016), pp.235-242.
https://search.emarefa.net/detail/BIM-726059
American Medical Association (AMA)
Fawzi, Amal& Swaifi, Karimah& al-Fayyumi, Hanin Muhammad& Nawfal, Najwa. Quantitative analysis of plasma cell-free DNA and its DNA integrity in patients with metastatic prostate cancer using ALU sequence. Journal of the Egyptian National Cancer Institute. 2016. Vol. 28, no. 4, pp.235-242.
https://search.emarefa.net/detail/BIM-726059
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 241-242
Record ID
BIM-726059