Antimicrobial, antioxidant activities and partial purification of ethyl acetate extract of Rosmarinus officinalis
Other Title(s)
فحص النشاطية المضادة للميكروبات و المضادة للأكسدة و التنقية الجزئية لمستخلص الإيثيل أسيتات لنبات إكليل الجبل
Dissertant
Thesis advisor
Comitee Members
Mustafa, Ahmad Husayn
al-Zaraiti, Wail Ali
al-Nuaymat, Sulayman
University
Mutah University
Faculty
Faculty of Science
Department
Department of Biological Sciences
University Country
Jordan
Degree
Master
Degree Date
2016
English Abstract
Antimicrobial and antioxidant activities of ethyl acetate extract from aerial parts of Rosmarinus officinalis were investigated.
The crude extract was partially purified using liquid column chromatography technique.
The bioactivity of crude extract and partially purified fractions was tested by using agar diffusion and minimum inhibitory concentration (MIC) assays as well as antioxidant assay using DPPH method.
Extract of R.
officinalis revealed antibacterial activity against Gram positive bacteria, namely, Bacillus subtilis, Micrococcus luteus and Staphylococcus aureus.
The crude extract caused an inhibition zone of 14.0 mm to B.
subtilis at 200μg/ml with MIC of (3 – 400 μg/ml) for all tested Gram positive bacteria.
The antioxidant activity of tested extract and partially purified fractions ranged from IC50 (26.124 -109.649 μg/ml).
They exhibited antioxidant activity with TEAC of (0.78 – 3.19) μg Trolox/ μg plant extract.
Therefore, R.
officinalis represents a good candidate for isolation of antimicrobial compounds and antioxidant agents with pharmaceutical significance.
Main Subjects
No. of Pages
54
Table of Contents
Table of contents.
Abstract.
Abstract in Arabic.
Chapter One : Theoretical background.
Chapter Two : Literature review.
Chapter Three : Design and methodology.
Chapter Four : Results, discussion and recommendation.
References.
American Psychological Association (APA)
al-Hijazin, Maysa Salim. (2016). Antimicrobial, antioxidant activities and partial purification of ethyl acetate extract of Rosmarinus officinalis. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-729039
Modern Language Association (MLA)
al-Hijazin, Maysa Salim. Antimicrobial, antioxidant activities and partial purification of ethyl acetate extract of Rosmarinus officinalis. (Master's theses Theses and Dissertations Master). Mutah University. (2016).
https://search.emarefa.net/detail/BIM-729039
American Medical Association (AMA)
al-Hijazin, Maysa Salim. (2016). Antimicrobial, antioxidant activities and partial purification of ethyl acetate extract of Rosmarinus officinalis. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-729039
Language
English
Data Type
Arab Theses
Record ID
BIM-729039
