Optimization of biodegradation conditions of 3-chlorobenzoic acid (3-CBA) by Acinitobacter calcoaceticus
Other Title(s)
الظروف المثلى للتحطيم الحيوي لمركب 3-كلوروبنزيك أسيد بواسطة بكتيريا Acinitobacter calcoaceticus
Dissertant
al-Samirrai, Ahmad Abd al-Hakim
Thesis advisor
Comitee Members
Ababinah, Ahmad
al-Zaraini, Wail Ali
al-Nuaymat, Sulayman Muhammad
University
Mutah University
Faculty
Faculty of Science
Department
Department of Biological Sciences
University Country
Jordan
Degree
Master
Degree Date
2015
English Abstract
Bacterium Acinitobacter calcoaceticus was isolated from agriculture soil of Al-mafraq governate - Jordan.
It was identified by using (16S ribosomal RNA).
This study was conducted to evaluate the ability of Acinitobacter calcoaceticus to degrade chlorobenzoic acid compounds especially 3-CBA and to evaluate the optimum condition for degradation.
Acinitobacter calcoaceticus was found to use the chlorobenzoate compounds as alternative carbon and energy source.
The percentages of degradation of the chrobenzoates 2-CBA, 3-CBA, 4-CBA and 3,4-dCBA at the concentration 2-mM were found after 80hr of incubation was 55%, 73%, 60.1% a and 54.9% respectively.
The order of initial rates were 30.3μM/h for (2-CBA), 20.6μM/h for (4-CBA), 10μM/h for (3,4-dCBA) and 55μM/h for (3-CBA).
The best percentage of degradation of 3-CBA compound were obtained with 3mM and 4mM substrate concentration and it was 73%.
Adaptation of the cells on 3-CBA for 48 hours and cells starvation for 24 hours and 48 hours increased significantly the initial degradation rate.
The carbon sources increased the cell biomass.
However, all carbon sources used reduced the biodegradation.
Nitrogen source reduced the degradation activity of the 3-CBA as well as the chlorine release from it.
Best inoculum size was 1.5xml.
Best agitation rate was 150RPM.
The experiments were included temperature and ph.
The preferential temperature and pH for the strain were 37ºC and ph.
0.7 respectively.
Main Subjects
No. of Pages
77
Table of Contents
Table of contents.
Abstract.
Abstract in Arabic.
Chapter One : Theoretical background.
Chapter Two : Literature review.
Chapter Three : Materials and method.
Chapter Four : Results and discussion.
References.
American Psychological Association (APA)
al-Samirrai, Ahmad Abd al-Hakim. (2015). Optimization of biodegradation conditions of 3-chlorobenzoic acid (3-CBA) by Acinitobacter calcoaceticus. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-729281
Modern Language Association (MLA)
al-Samirrai, Ahmad Abd al-Hakim. Optimization of biodegradation conditions of 3-chlorobenzoic acid (3-CBA) by Acinitobacter calcoaceticus. (Master's theses Theses and Dissertations Master). Mutah University. (2015).
https://search.emarefa.net/detail/BIM-729281
American Medical Association (AMA)
al-Samirrai, Ahmad Abd al-Hakim. (2015). Optimization of biodegradation conditions of 3-chlorobenzoic acid (3-CBA) by Acinitobacter calcoaceticus. (Master's theses Theses and Dissertations Master). Mutah University, Jordan
https://search.emarefa.net/detail/BIM-729281
Language
English
Data Type
Arab Theses
Record ID
BIM-729281
