Spectrophotometric determination of bisacodyl in pure and pharmaceutical preparation via oxidative coupling organic reaction

Abstract EN

A simple, accurate and sensitive spectrophotometric way is used to determine Bisacodyl in pure and pharmaceutical preparations.

The proposed method depends on using 2,4-Dinitrophenylhydrazine as chromogenic reagent.

The method was based on the oxidative coupling reaction of Bisacodyl with 2,4-Dinitrophenylhydrazine with Sodium periodate in the presence of sodium hydroxide as alkaline media to form red water soluble dye product , that has a maximum absorption at Xmax 522nm .

Beer ,s law is obeyed in the concentration of (2.00-20.00) pg.ml "1 .The molar absorptivity is (6505) L.mol-1.cm-1,a sandall sensitivity of(0.0555) pg.cm"2), correlation coefficient of (0.9970), Limitof detection (LOD) (0.0312 pg.ml"1), limit of Quantitation (LOQ) (0.

3125 pg.ml"1) and the relative standard deviation of RSD% (1.6).

The method gave a successful determination for Bisacodyl in pharmaceutical preparations and the value of recovery % was better than (100.16%).