Evaluation of silver nanoparticles (Ag NPs) activity against the viability of Leishmania tropica promastigotes and amastigotes in vitro
Other Title(s)
تقدير فعالية الفضة المتناهية الصغر على حيوية الطور أمامي السوط و عديم السوط للشمانيا الإستوائية في المختبر
Joint Authors
Gharbi, Miad Abd al-Razzaq
Jafar, Sadiq Mawla
al-Qadi, Ban Nuri
Source
Issue
Vol. 58, Issue 1A (31 Mar. 2017), pp.13-21, 9 p.
Publisher
University of Baghdad College of Science
Publication Date
2017-03-31
Country of Publication
Iraq
No. of Pages
9
Main Subjects
Topics
Abstract EN
Leishmaniasis is a disease caused by a protozoan parasite of the genus Leishmania.
It is transmitted by the bite of sandfly (Subfamily Phlebotominae).
Limited drugs are available for the treatment of leishmaniasis, and the general drug (pentostam) have many side effect on patients.
Therefore, there is an urgent need for another drugs for the treatment of leishmaniasis.
This study aimed to develop new type of antileishmanial agents instead of classical drug (pentostam) and investigated the effectiveness of silver nanoparticles (Ag NPs) on Leishmania tropica parasites in both phases promastigote and amastigote in comparision to pentostam in in vitro condition.
This study showed the effects of Ag NPs in comparision to pentostam with different concentrations (0.3, 0.6, 0.9, 1.2, 1.5, 1.8 and 2.1 ^g/ ml) on L.tropica promastigotes viability.
The viability of promastigotes after 72 hr.
recorded maximum cytotoxic effect of Ag NPs in highest concentration (2.1 ^g/ml), it was 23.17 ± 0.45 % comparing with pentostam which was 69.33 ± 0.33 %, as well as IC50 was calculated for MTT assay and the result for Ag NPs was 1.
749 ^g/ml after 72 hr., while pentostam drug did not show IC50 in all treatments.
On the other hand, the study also showed the effects of Ag NPs on L.tropica amastigote phase, and the viability was (3.10 ± 0.59 and 47.34 ± 0.87) % after 72 hr.
in the highest concentration 2.1 ^g/ml for Ag NPs and pentostam respectively, and IC50 was 1.148 ^g/ ml for Ag NPs after 72 hr., but all results of pentostam stayed over than 50%.
American Psychological Association (APA)
Gharbi, Miad Abd al-Razzaq& al-Qadi, Ban Nuri& Jafar, Sadiq Mawla. 2017. Evaluation of silver nanoparticles (Ag NPs) activity against the viability of Leishmania tropica promastigotes and amastigotes in vitro. Iraqi Journal of Science،Vol. 58, no. 1A, pp.13-21.
https://search.emarefa.net/detail/BIM-732570
Modern Language Association (MLA)
Gharbi, Miad Abd al-Razzaq…[et al.]. Evaluation of silver nanoparticles (Ag NPs) activity against the viability of Leishmania tropica promastigotes and amastigotes in vitro. Iraqi Journal of Science Vol. 58, no. 1A (2017), pp.13-21.
https://search.emarefa.net/detail/BIM-732570
American Medical Association (AMA)
Gharbi, Miad Abd al-Razzaq& al-Qadi, Ban Nuri& Jafar, Sadiq Mawla. Evaluation of silver nanoparticles (Ag NPs) activity against the viability of Leishmania tropica promastigotes and amastigotes in vitro. Iraqi Journal of Science. 2017. Vol. 58, no. 1A, pp.13-21.
https://search.emarefa.net/detail/BIM-732570
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 20-21
Record ID
BIM-732570