In vitro assessment of miltefosine activity against promastigotes and axenic amastigotes of Leishmania tropica

Other Title(s)

تقييم فعالية الملتيفوسين ضد الطور أمامي السوط و عديم السود الخارجي للشمانيا الإستوائية داخل المختير

Author

al-Rawi, Khawlah Hawari Zughayr

Source

Iraqi Journal of Science

Issue

Vol. 58, Issue 1A (31 Mar. 2017), pp.22-30, 9 p.

Publisher

University of Baghdad College of Science

Publication Date

2017-03-31

Country of Publication

Iraq

No. of Pages

9

Main Subjects

Medicine
Pharmacology

Topics

Abstract EN

Leishmaniasis is a worldwide disease still treated with expensive compounds that present severe side effects, and are frequently ineffective emphasizing the importance to search effective compounds against this disease.

Miltefosine drug (HePC) that used as antitumor agent has been used against Leishmania tropica in two forms promastigote and axenic amastigote in vitro conditions.

Different concentrations (5, 10, 15, 20, 25 and 30 ^M) of HePC were performed and exposed to both parasite forms in comparison to sodium stibogluconate (Sb) drug.

Parasites viability then was determined using MTT assay after 12, 24, and 48hr of exposure.

DNA was extracted from treated and untreated parasites after 48hr of exposure and qualitative analysis of the total genomic DNA fragmentation was carried out through using agarose gel electrophoresis.

The IC50 of HePC and Sb were 17.07 and 25.72 ^M against L.

tropica promastigotes, respectively, while, the IC50 of them were 18.08 and 21.29^M against axenic amastigotes respectively.

Fragmented DNA in agarose gel electrophoresis was revealed in both parasite forms exposed to all used concentrations of HePC.

These results showed the significant activity of miltefosine against the viability of L.

tropica promastigotes and axenic amastigotes.

American Psychological Association (APA)

al-Rawi, Khawlah Hawari Zughayr. 2017. In vitro assessment of miltefosine activity against promastigotes and axenic amastigotes of Leishmania tropica. Iraqi Journal of Science،Vol. 58, no. 1A, pp.22-30.
https://search.emarefa.net/detail/BIM-732601

Modern Language Association (MLA)

al-Rawi, Khawlah Hawari Zughayr. In vitro assessment of miltefosine activity against promastigotes and axenic amastigotes of Leishmania tropica. Iraqi Journal of Science Vol. 58, no. 1A (2017), pp.22-30.
https://search.emarefa.net/detail/BIM-732601

American Medical Association (AMA)

al-Rawi, Khawlah Hawari Zughayr. In vitro assessment of miltefosine activity against promastigotes and axenic amastigotes of Leishmania tropica. Iraqi Journal of Science. 2017. Vol. 58, no. 1A, pp.22-30.
https://search.emarefa.net/detail/BIM-732601

Data Type

Journal Articles

Language

English

Notes

Includes bibliographical references : p. 29-30

Record ID

BIM-732601