Synergistic effect of biosynthesized silver nanoparticles with antibiotics against multi-drug resistance bacteria isolated from children with diarrhoea under five years
Other Title(s)
التأثير التأزري لدقائق الفضة النانوية المصنعة حيويا من المضادات الحيوية ضد البكتريا متعددة المقاومة للعقار و المعزولة من الأطفال المصابين بالإسهال بعمر أقل من خمس سنوات
Joint Authors
al-Ukaydi, Isra Ali Zaydan
Tawfiq, Shahd Maʻad
Maruf, Muhammad Nazir
Source
Issue
Vol. 58, Issue 1A (31 Mar. 2017), pp.41-52, 12 p.
Publisher
University of Baghdad College of Science
Publication Date
2017-03-31
Country of Publication
Iraq
No. of Pages
12
Main Subjects
Topics
Abstract EN
Isolation and identification of bacterial isolates were carried out according to the morphology and biochemical characteristics on one hundred and twenty stool specimens collected from children under five years old via using biochemical tests and Api 20E compact system for further confirmation.
Bacterial isolates were distributed as (34.48, 20.68, 5.17,0.86) % for Escherichia coli, Salmonella typhi ,Enterobacter aerogenos, Citrobacter freundii and Hafnia alvei respectively and 9.48 % for each Proteus mirabilis, Pseudomonas aeruginosa and Klebsiella Pneumonia.
As well as, 2.58% for both Shigella sonnei and Serratia marcescens.
Antibiotic susceptibility test for 116 bacterial isolates was performed towards 20 antibiotics types using disk diffusion method.
The results showed dissimilar resistance values towards different antibiotics, ten bacterial isolates were collected for each bacterial species to study their resistance values, the ones with the highest resistance level were selected for further study.
Meanwhile, easy and cheap green method using the banana peel extract (BPE) was applied to synthesize silver nanoparticles (AgNPs).
Phytochemicals of BPE were screened by standard methods.
The results verified the existence of alkaloids, flavonoids, and glycosides in it.
These components were act as a reducing agent ,stabilizing and capping agents for AgNO3 with the assistance of the microwave.
The successfully preparation of AgNPs was established by ultraviolet-visible spectroscopy, Transmission electron microscopy (TEM), Dynamic light scattering (DLS), Fourier Transform Infrared Spectroscopy (FTIR) and zeta potential analysis.
The antibacterial activity of the AgNPs against multidrug resistance (MDR) bacteria were studied by using disk diffusion method.
The results showed a considerable effect against MDR isolates.
The synergistic effects of biosynthesis AgNPs at different concentrations with different standard antibiotic discs (which were Tobramycin, Chloramphenicol, Nitrofuration, Ampicillin-clavulanic acid and Nalidixic acid) against MDR bacteria were also investigated.
The result showed the synergistic action of AgNPs and antibiotics leading to enhance antibacterial activity.
American Psychological Association (APA)
Tawfiq, Shahd Maʻad& Maruf, Muhammad Nazir& al-Ukaydi, Isra Ali Zaydan. 2017. Synergistic effect of biosynthesized silver nanoparticles with antibiotics against multi-drug resistance bacteria isolated from children with diarrhoea under five years. Iraqi Journal of Science،Vol. 58, no. 1A, pp.41-52.
https://search.emarefa.net/detail/BIM-732653
Modern Language Association (MLA)
Tawfiq, Shahd Maʻad…[et al.]. Synergistic effect of biosynthesized silver nanoparticles with antibiotics against multi-drug resistance bacteria isolated from children with diarrhoea under five years. Iraqi Journal of Science Vol. 58, no. 1A (2017), pp.41-52.
https://search.emarefa.net/detail/BIM-732653
American Medical Association (AMA)
Tawfiq, Shahd Maʻad& Maruf, Muhammad Nazir& al-Ukaydi, Isra Ali Zaydan. Synergistic effect of biosynthesized silver nanoparticles with antibiotics against multi-drug resistance bacteria isolated from children with diarrhoea under five years. Iraqi Journal of Science. 2017. Vol. 58, no. 1A, pp.41-52.
https://search.emarefa.net/detail/BIM-732653
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 49-52
Record ID
BIM-732653