Diagnostic tests for detection of bovine tuberculosis in dairy cattle farms compared to tuberculin test

Abstract EN

A total number of 143 cows and buffaloes cases in Qena and El-Menoufya Governorates were examined for bovine tuberculosis using single intradermal cervical tuberculin test (SICTT).

109 out of 143 (76.22%) tested cattle were found positive to tuberculin test.

Tissue samples from organs positive cases to tuberculin test were examined microscopically with Ziehl-neelsen stain and by cultivation on two different types of media (Middlebrook and Lowenstein Jenssen media).

Blood serum samples from the positive cases were tested by ELISA.

ELISA plates were coated with bovine purified protein derivative (PPD) and short term culture filtrate (ST-CF) antigens.

The test sensitivity was compared at different serum dilutions.

The result was 102 out of 109 cases were positive by cultivation technique with a percentage of (71.32%) and 94 out of 109 cases were positive by microscopical examination with a percentage of (65.73%).

The results of ELISA and „ traditional cultural methods as well as tuberculin test were compared ana j discussed.

All serum samples of tuberculin positive cow cases were | tested by indirect ELISA, and showed that 26 out of 31 cow sera werel positive by PPD with a percentage of (83.87%) and 29 out of 31 cases! were positive by using ST- CF antigens with a percentage of (93.5%) inl (1\40) serum dilution.

Concerning buffalo cases, 71 out of 78 tuberculirJ positive sera were positive with PPD antigen with a percentage of (91%jjj and 77 sera cases were positive with ST- CF antigen with a percentage! of (98.7%) at serum dilution (1\40).

Regarding the tuberculin negative cases, out of 16 tuberculin negative cow cases there were 9 positive! cases by using PPD antigen with a percentage of (56.25%) and.:.|3 positive cases by using ST- CF antigen with a percentage of (37.5%W Concerning the buffalo cases, there were 3 positive cases out of Ja tuberculin negative sera by using PPD antigen with a percentage^ (16.66%) and one case was positive by using ST- CF antigen withjg percentage of (5.55%).