Microbiological quality of soaps and efficacy of antiseptics and disinfectants used in hospitals in Gaza-Palestine

Other Title(s)

الجودة الميكروبية للصابون و فعالية المطهرات المستخدمة في مستشفيات قطاع غزة-فلسطين

Dissertant

Salamah, Ahmad Salih Awdah

Thesis advisor

al-Manamah, Abd al-Rauf Ali

Comitee Members

Shubayr, Muhammad Id
Abu al-Khayr, Imad Khalil

University

Islamic University

Faculty

Faculty of Science

Department

Department of Biological Science

University Country

Palestine (Gaza Strip)

Degree

Master

Degree Date

2016

English Abstract

The aim of the present study is a determine microbiological (bacteria and fungi) quality of antiseptics and soap samples, identify bacteria that contaminate antiseptics and soaps that used in hospitals in Gaza – Palestine, measure the efficacy of antiseptics on bacteria, and determine the chemical efficacy of antiseptics and disinfectants.

To determine microbiological (bacteria and fungi) quality of antiseptics and soap samples and identify bacteria that contaminate antiseptics and soaps that used in hospitals in Gaza – Palestine, I used plated media method, to measure the efficacy of antiseptics on bacteria, I used stainless steel cylinder method, and to determine the chemical efficacy of antiseptics and disinfectants I used pH measurements and chemical concentration test.

The soap results shown as the percentage of samples that complied with the standards (passed) was 15/15 (100%) in European Gaza Hospital, and the lowest passing result as 1/14 (6.7%) in Kamal Adwan Hospital and the total passing value as 73/105 (69.5%) and the total failing value as 32/105 (30.5%), and The results showed that the percentage of contaminated samples by bacteria and fungi was 18/105 (17.1%) and total percentage value was 32/105 (30.5%), the most common contaminant was coliform 13/105 (13.4%), Pseudomonas spp.

12/105 (11.4%) and Bacillus spp.

6/105 (5.7%).

The contamination with yeast 11/105 (10.5%) was more than the mold 7/105 (6.7%), and The pH test results showed that the average of pH of soap samples in hospitals indicate the highest passing value as 18/20 (90%) in Nasser Hospital, the lowest passing value as 0/15 (0%) in European Gaza hospital, and the total passing value as 43/105 (41%), and the total failing value as 62/105 (59%).

The antiseptics/disinfectants results shown as the average of zone of inhibition is 28.2 mm for E.

coli, 20.8 mm for P.

aeruginosa, and 20.7mm for S.

aureus, the total percentage passing samples by concentration is 140/233 (60.09%) and the percentage of failing sample in all hospitals is 93/233 (39.91%), and the highest percentage of passing results is 79.66% for Chlorhexidine, and lowing percentage of passing results is 48.28% for Chlorine, and in pH parameter that total percentage of passing results is 141/144 (97.9%), and that the total percentage of falling results is 3/144 (2.1%).

In conclusions, the results of the present study show that more than half samples collected from biggest central hospital in Gaza stripe failed in tests, and the percentage of fail in Antiseptics/Disinfectants samples were 40.3%, and in soap samples were 74.3%.this results may become a potential human health hazard, the main causes were using locally manufactured soaps and disinfectants and over dilution the antiseptics/disinfectants before using.

Main Subjects

Biology

No. of Pages

89

Table of Contents

• APA
• MLA
• AMA

Language

English

Data Type

Arab Theses

Record ID

BIM-735237