التشكل الوراثي لجين الكالبستاتين في الأبقار المحلية و الجاموس العراقي

Abstract EN

Data of the present study collected from the slaughter house of Dhi-Qar Governorate.

These data represent 110 samples from Janoubi bull aged 2-3 year and 50 samples from Iraqi buffalo bulls at the same age.

From both cattle and buffalo animal 10ml of blood were taken to extract DNA.

Calpastatin primer was amplified and digests using PCR-RFLP method and MspI restriction enzyme.

PAGE electrophoresis was used to determine genotypes.

Calpastatin primer showed single band of 622bp from the first exon and part of first intron of the gene.

Two alleles M and N appeared with three genotypes, MM with two bands, MN with three bands and NN with one band in both cattle and buffalo.

Allele frequencies were 0.69 for the M allele in cattle and 0.27 in buffalo.

Those of N allele were 0.31 and 0.73 respectively.

Genotype frequencies of MM, MN and NN were 0.46, 0.45 and 0.09 respectively in cattle and 0.08, 0.38 and 0.54 respectively in buffalo.

HW test indicated both populations were under Hardy-Weinberg equilibrium.

Genetic analysis revealed the lack of inbreeding within cattle population and very low percentage (1.8 %) of inbreeding within buffalo population.