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Study of some immunological parameters in Iraqi patients with multiple sclerosis
Other Title(s)
دراسة بعض المعالم المناعية لمرضى التصلب المتعدد العراقيين
Dissertant
al-Faradhi, Hind Qusayy Ismail
Thesis advisor
University
University of Baghdad
Faculty
College of Education for Puresciences-IBN al-Haitham
Department
Department of Biology
University Country
Iraq
Degree
Master
Degree Date
2015
English Abstract
Multiple sclerosis (MS) is an inflammatory condition that affects central nervous system (CNS) causing neurological dysfunction.
The current study aim was the evaluation of the role of Methylprednisolone (MP), Interferon Beta (IFN-β) as disease treatments, and to investigate their influence on CD25+ FoxP3 T regulatory cells (it was counted by flow cytometry technique), IL-8, IL-17 (pro-inflammatory cytokines), IL-12 (inflammatory cytokine (T cells differentiation Factor)), IL-10, TGF-β (anti-Inflammatory cytokines) it estimated by ELISA technique, also the total and differential WBC count, ESR, CRP (inflammatory indicators), patient characteristics, clinical manifestation and life style characteristics.
Forty-five Iraqi MS patients (15 untreated as early onset patients, 15 MP treated patients and 15 IFN-β treated patients), in addition to 15 apparently healthy individuals as control.
For patients treated with MP, samples collected after treatment period, while patients treated with IFN-β samples were collected in remission status.
The study revealed the following results: 1.
Patient general characteristics showed that according to gender distribution female:male ratio was 2:1 (66.67% : 33.33%), according to the age of onset, (< 30 years) group of patients were the highest frequency in study sample (37.9%), 57 years was the maximum age of onset and 13 years was the minimum, with mean (34.93), Baghdad was the highest governorate in MS onset (73.33%).
2.
Total WBC count showed that patients treated with MP (10.38*103μl) increases significantly (P≤0.002), (P≤0.001) as compared with both patients treated with IFN-β (7.45*103μl) and control (7.33*103μl) respectively.
Lymphocytes were decrease INF-β respectively (1.82), (1.92) as compared to control (2.58) and there were a significant decrease (P≤0.03) in patients treated with MP (1.82*103μl) as compared to untreated patients (2.47*103μl).
Neutrophils was increase significantly (P≤0.016) and (P≤0.000) in patients treated with MP and untreated patients respectively (7.81), (5.99) as compared to control (3.82), there were also a significant increase in patients treated with MP (P≤0.04), (P≤0.001) as compared with untreated patients (5.99*103μl) and patients treated with IFN-β (4.73*103μl) respectively.
Basophils decrease significantly (P≤0.001), (P≤0.014) in both patients treated with MP and INF-β respectively (0.05), (0.06) as compared to control (0.08).
3.
CD25+ FoxP3+ Tregs counting showed a significant increase (P≤0.002) in untreated patients (28.01%) as compared to control (14.06%), while there were a significant decrease (P≤0.016), (P≤0.007) in both patients treated with MP and IFN-β respectively (3.70%), (2.44%) as compared to control, also there were a significant increase (P≤0.000), (P≤0.000) in untreated patients (28.01%) as compared with patients treated with MP (3.70%) and patients treated with IFN-β (2.44%) respectively.
4.
Serum cytokines estimation showed that IL-8 decreased significantly (P≤0.026) in untreated patients (0.003pg/ml) as compared to control (0.016), while IL-10, IL-12, IL-17 showed no significances and TGF-β increased significantly (P≤0.028) in patients treated with MP (700.27) as compared to control (392.13), while there were a significant increasing (P≤0.001), (P≤0.001) in patients treated with MP (700.27pg/ml) as compared with untreated patients (235pg/ml) and patients treated with IFN-β (484.67pg/ml) respectively.
Saliva cytokines estimation showed that IL-8 was increased significantly (P≤0.023) in patients treated with IFN-β (0.578) as compared to control (0.223), IL-10 increased significantly (P≤0.017) in patients treated with MP (0.023) as compared to control (0.00), IL-12 decreased significantly (P≤0.003) in patients treated with IFN-β (4.93) as compared to control (24.07), IL-17 increased significantly (P≤0.018) in patients treated with MP (19.17) as compared to control (0.00).
Main Subjects
No. of Pages
114
Table of Contents
Table of contents.
Abstract.
Abstract in Arabic.
Chapter One : Introduction.
Chapter Two : Literature review.
Chapter Three : Subjects, materials and methods.
Chapter Four : Results and discussion.
Conclusions and recommendations.
References.
American Psychological Association (APA)
al-Faradhi, Hind Qusayy Ismail. (2015). Study of some immunological parameters in Iraqi patients with multiple sclerosis. (ماجستير Theses and Dissertations Master). جامعة بغداد, Iraq
https://search.emarefa.net/detail/BIM-739639
Modern Language Association (MLA)
al-Faradhi, Hind Qusayy Ismail. Study of some immunological parameters in Iraqi patients with multiple sclerosis. (ماجستير Theses and Dissertations Master). جامعة بغداد. (2015).
https://search.emarefa.net/detail/BIM-739639
American Medical Association (AMA)
al-Faradhi, Hind Qusayy Ismail. (2015). Study of some immunological parameters in Iraqi patients with multiple sclerosis. (ماجستير Theses and Dissertations Master). جامعة بغداد, Iraq
https://search.emarefa.net/detail/BIM-739639
Language
English
Data Type
Arab Theses
Record ID
BIM-739639