خبن الصبغي Y لدى مرضى انعدام النطاف و علاقته مع الإنطاف
Other Title(s)
Y-chromosome microdeletion in azoospermic patients and its relationship with spermatogenesis
Author
Source
Issue
Vol. 30, Issue 1 (30 Jun. 2014), pp.11-22, 12 p.
Publisher
Publication Date
2014-06-30
Country of Publication
Syria
No. of Pages
12
Main Subjects
Abstract EN
Background& Objective: Microdeletions of the Y-chromosome removing the azoospermia factor (AZF) region are the most frequent molecular genetic cause of spermatogenic failure.
Although there is still no definitive consensus on the relationship between the type of microdeletion and the resulting sperm defect, microdeletions in AZFa lead mostly to Sertoli cell only syndrome, mutations in AZFb provoke interruption in meiosis I and mutations in AZFc result in hypospermatogenesis.
Our objective is to evaluate the frequency and types of Y-chromosome microdeletions in patients with idiopathic nonobstructive azoospermia (NOA) and its relationship with successful sperm retrieval.
Materials & Methods: Study design: cross sectional study.
Participants: 393 males with NOA.
Duration: from February 2009 to February 2012.
Setting: Department of anatomy, histology and embryology – Damascus University, and Orient Hospital.
Clinical Intervention(s): Semen analysis, karyotype, Y-chromosome microdeletion testing by polymerase chain reaction (PCR) and testicular sperm extraction (TESE).
Results: The frequency of Y-chromosome microdeletions was 14% (55/393), and microdeletion in the AZFb region were the most prevalent 49.1% (27/55), followed by the AZFc, ADFd and AZFa.
Spermatozoa were retrieved in 11 cases (57.9%) of microdeletions in AZFc region, and were absent in all other microdeletions.
Therefore, the presence of an AZFc microdeletion was associated with increased likelihood of sperm retrieval.
Conclusion: The high prevalence of Y-chromosome microdeletions (14%) in our study strongly suggests the need for routine molecular genetic testing and counseling prior to assisted reproduction in idiopathic NOA, interestingly the successful sperm retrieval was only found in microdeletions located in sub-region of azoospermia factor (AZFc).
American Psychological Association (APA)
الحلبي، مروان. 2014. خبن الصبغي Y لدى مرضى انعدام النطاف و علاقته مع الإنطاف. مجلة جامعة دمشق للعلوم الطبية،مج. 30، ع. 1، ص ص. 11-22.
https://search.emarefa.net/detail/BIM-743265
Modern Language Association (MLA)
الحلبي، مروان. خبن الصبغي Y لدى مرضى انعدام النطاف و علاقته مع الإنطاف. مجلة جامعة دمشق للعلوم الطبية مج. 30، ع. 1 (2014)، ص ص. 11-22.
https://search.emarefa.net/detail/BIM-743265
American Medical Association (AMA)
الحلبي، مروان. خبن الصبغي Y لدى مرضى انعدام النطاف و علاقته مع الإنطاف. مجلة جامعة دمشق للعلوم الطبية. 2014. مج. 30، ع. 1، ص ص. 11-22.
https://search.emarefa.net/detail/BIM-743265
Data Type
Journal Articles
Language
Arabic
Notes
يتضمن مراجع ببليوجرافية : ص. 20-22
Record ID
BIM-743265