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The effect of storage temperature on the surface free energy of spray dried lysozyme powders
Other Title(s)
تأثير حرارة التخزين في طاقة السطح الحرة لمساحيق الليزوزيم المجفف بالإرذاذ
Source
Damascus University Journal for Medical Sciences
Issue
Vol. 30, Issue 2 (31 Dec. 2014), pp.149-156, 8 p.
Publisher
Publication Date
2014-12-31
Country of Publication
Syria
No. of Pages
8
Main Subjects
Abstract EN
Background & Objective: Spray drying techniques are used to produce inhaled powders of enzymes and proteins without denaturation.
The surface free energy data of these powders is used to reduce the time of formulation development.
During storage, the surface free energy of the inhaled protein powders could change and so their performance will be affected.
Many factors affect the limit of this change.
Storage temperature is the critical factor.
Material& methods: A spray drying method was optimized to prepare inhaled spray dried lysozyme powders.
Inverse gas chromatography was utilized to measure the surface free energy of the prepared powders pre and post-storage to detect the effect of storage temperature on the surface free energy.
Differential Scanning Calorimetry and biological activity tests were used to evaluate the integrity of lysozyme conformation after storage at different storage temperature.
Results: Increasing the storage temperature decreased both dispersive and specific surface components of the spray-dried lysozyme powders significantly (ANOVA: P < 0.001) up to ~12% and up to ~31 % after storage for one year at 40 °C, respectively.
The overall decrease in the polar components resulted in a reduction of the surface basicity.
These decreases in the surface energetics would be due to the aggregation of denatured lysozyme molecules at the powder surface via hydrophobic regions rich with electrons.
The same results were found after storage for one year at room temperature, but the extents of changes were smaller.
Conclusion: It is advisable to store protein powders at 5°C to inhibit the changes in their surface free energy which is the crucial factor affecting their performance.
American Psychological Association (APA)
2014. The effect of storage temperature on the surface free energy of spray dried lysozyme powders. Damascus University Journal for Medical Sciences،Vol. 30, no. 2, pp.149-156.
https://search.emarefa.net/detail/BIM-743371
Modern Language Association (MLA)
The effect of storage temperature on the surface free energy of spray dried lysozyme powders. Damascus University Journal for Medical Sciences Vol. 30, no. 2 (2014), pp.149-156.
https://search.emarefa.net/detail/BIM-743371
American Medical Association (AMA)
The effect of storage temperature on the surface free energy of spray dried lysozyme powders. Damascus University Journal for Medical Sciences. 2014. Vol. 30, no. 2, pp.149-156.
https://search.emarefa.net/detail/BIM-743371
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 155-156
Record ID
BIM-743371