Role of autologous platelets rich plasma in treatment of experimentally induced Achilles tendonitis in rabbits

Abstract EN

The study aimed to a clinico-histopathological evaluating the benefit of using autologous platelets rich plasma (PRP) in healing of Achilles tendonitis in rabbits.

Twenty adult male rabbits were used.

Five ml of blood was withdrawn from the rabbits marginal ear vein and mixed with sodium citrate for preparation of PRP.

Rabbits were randomly allocated into two groups (10 of each).

The first group, serve as a control group and the second group, considered as treatment group.

Tendonitis was induce under the effect of general anesthesia.

Lateral longitudinal incision on the skin over the Achilles tendon was made.

The tendon was isolated by blunt dissection from the surrounding tissue.

Tendonitis was induced by splitting of the tendon with surgical blade.

The first group (treated with one ml normal saline).

In contrast, the second group (treated with one ml of PRP).

Both saline and PRP were injected intra-lesional after that the surgical skin wounds was re stitched in routine manner.

After clinical follow-up of the treatment rabbits, certain secondary complications were happened represented by lameness, swelling and infection.

Histo-pathological evaluation was performed at 8 and 16 weeks post-surgery (10 rabbits/group) (5 rabbits/period).

Grossly, adhesion was noticed in most rabbits of control group.

Microscopical examination reflect perfect orientation and organization of collagen fibers in treatment group in comparing with control group.

Based on the results obtain from this study, PRP enhanced and promote tendon healing.