Molecular and serological study by using ELISA and rRT-PCR techniques to detect avian infectious bronchitis virus in chickens in middle Euphrates
Other Title(s)
دراسة جزيئية و مصلية باستخدام اختباري الإليزا و تفاعل سلسلة البلمرة المنعكس للكشف عن فايروس التهاب القصبات المعدي في الدواجن في الفرات الأوسط
Joint Authors
Shayyal, Zaynab Kazim
Abd, Ala Abd al-Aziz
Source
al-Qadisiyah Journal of Veterinary Medicine Sciences
Issue
Vol. 13, Issue 1 (30 Jun. 2014), pp.25-31, 7 p.
Publisher
al-Qadisiyah University College of Veterinary Medicine
Publication Date
2014-06-30
Country of Publication
Iraq
No. of Pages
7
Main Subjects
Abstract EN
The study was conducted to detect Infectious Bronchitis Virus (IBV) in commercial chicken farms of Middle Euphrates, the technique have been used throughout the study protocol, Enzyme Linked Immunosorbent Assay (ELISA), Real Time Reverse Transcription Polymerase Chain Reaction (RT-PCR) and histopathological study.
(172) serum samples were collected from clinically infected vaccinated broiler farm and (29) serum samples were collected from clinically infected non-vaccinated broiler farm, and screened for the presence of IBV antibodies by ELISA.75serum samples from 172 samples were positive ,(43.6%) distributed as followed 43 (61.4%) from Hella, 23 (36.5%) from Najaf and 9 (23.07%) from Diwaneyah ,29 serum samples collected from clinically infected non-vaccinated broiler flocks, were 5 (17.2%) serum positive samples for IBV.
(75) tissue samples from clinically infected vaccinated broilers distributed were submitted for rRT-PCR technique, the results were as followed42 (56%) have been detected IBV, while 30 (71.4%) was positive in ELISA, and (15) samples from clinically infected non-vaccinated broilers showed 8 (53.3%) IBV were detectable while 5 (62.5%) was positive in ELISA.
Also the research include study the histopathological changes of trachea, lung and kidney of infected birds which were positive for rRT-PCR.
American Psychological Association (APA)
Abd, Ala Abd al-Aziz& Shayyal, Zaynab Kazim. 2014. Molecular and serological study by using ELISA and rRT-PCR techniques to detect avian infectious bronchitis virus in chickens in middle Euphrates. al-Qadisiyah Journal of Veterinary Medicine Sciences،Vol. 13, no. 1, pp.25-31.
https://search.emarefa.net/detail/BIM-743472
Modern Language Association (MLA)
Abd, Ala Abd al-Aziz& Shayyal, Zaynab Kazim. Molecular and serological study by using ELISA and rRT-PCR techniques to detect avian infectious bronchitis virus in chickens in middle Euphrates. al-Qadisiyah Journal of Veterinary Medicine Sciences Vol. 13, no. 1 (2014), pp.25-31.
https://search.emarefa.net/detail/BIM-743472
American Medical Association (AMA)
Abd, Ala Abd al-Aziz& Shayyal, Zaynab Kazim. Molecular and serological study by using ELISA and rRT-PCR techniques to detect avian infectious bronchitis virus in chickens in middle Euphrates. al-Qadisiyah Journal of Veterinary Medicine Sciences. 2014. Vol. 13, no. 1, pp.25-31.
https://search.emarefa.net/detail/BIM-743472
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 30-31
Record ID
BIM-743472