Histopathological, cytogenetical and fine structure study for the role of selenium and olive oil against originated lead toxicity in female mice (Mus musculus L.)‎

Other Title(s)

دراسة نسجيه مرضية و وراثية و تركيبية دقيقة لدور السلينيوم و زيت الزيتون ضد التسمم المستحدث بالرصاص في إناث الفئران (Mus musculus L.)‎

Dissertant

al-Khalid, Wala Jamil Muslim

Thesis advisor

Husayn, Adil Jabbar
Majid, Majdi Faysal

University

University of Basrah

Faculty

Veterinary College

Department

Department of Anatomy

University Country

Iraq

Degree

Master

Degree Date

2013

English Abstract

The present study was conducted at Veterinary Medicine College – University of Basrah .The cell structure, cellular cytogenetic and histopathological changes in the brain, liver, lung, kidney of adult female mice exposed to different doses of lead acetate and the therapeutic role of selenium and olive oil as an antioxidant.

Seventy nine female mice, 12 weeks old, and of 20–25 grams weight were used.

This study included two experimental designs.

The first experiment involved seven groups, each group consisted of seven female mice, the control group was orally given 0.9 % normal saline daily for 28 days, While the treated groups A,B,C,D,E,F received orally 50 mg/kg B.W.

lead acetate, 50 mg/kg of lead acetates + o.5 mg/kg of selenium, 50 mg/kg of lead acetates +0.2 CC of olive oil, 100 mg/kg of lead acetates, 100 mg/kg of lead acetates+ o.5 mg/kg of selenium, 100 mg/kg of lead acetates+0.2 CC of olive oil daily for28 days, respectively.

The measured parameter obtain during 7,14,21,and 28 days of the experiment.

The concentration of some hemo metals, histopathological and cytogenetic changes studies were done after oral administration.

In the secondary experimental design, the animals were divided into three group each groups consisted of 10 mice, the control group was orally given 0.9 % normal saline.

While the treated group A received orally 50 mg/kg B.W.

lead acetate for 21 days and after that it was treated with selenium and olive oil for 21 days too .while group B received orally 100 mg/kg BW lead acetate for 21 days and after that it was treated with selenium and olive oil for 21 days, too.

The ultra structure, histopathological examinations were done.

The results of the study revealed that the liver had the highest values of lead concentration in both doses of lead acetate in compared to other organs (kidney, lung and spleen).

While, the results recorded decrease of lead concentration in all organs after treated with Se and O.O.

Furthermore, the highest value of lead accumulation was recorded at the dose of lea100 mg/kg at the 28 day of exposure time.

Anatomical examination of the treatment groups showed increase in the volume of liver and spleen (hepatomegaly , spleenomegaly) , sever congestion in the liver, heart and lungs.

Stomach and intestine appeared swelled especially large intestine, on the other hand, the oral administration of olive oil caused mild decrease in some of anatomical changes like mild congestion and mild decrease in liver and spleen volume, whereas the selenium caused disappearance of the anatomical changes that were like normal structure.

Cytological (celluler) examination revealed changes in hepatocytes that were represented by certain damages to some organelles included electron lucent cytoplasm, heterochromatic nuclei , degeneration of rough endoplasmic reticulum ,fat globules , irregular short microvilli, ill-defined cell boundaries ,accumulation of vacules in the cytoplasm and poorly identifiable organelles ,apoptotic body in parts of nucleus, hemolysis and necrosis on lysosomes , megamitochondrion with longitudinal crystal formations.

On the other hand, the treatment with selenium and olive oil showed decreased in these changes.

Cytogenetic examination showed that the administration of lead caused significant increase (P ≤0.05) in number of chromosomal aberration contain of (ring chromosome, fragmentation, centric fusions and centromeric attenuation ) .The high dose 100 mg/ kg caused more increase in number of chromosomal aberration at 28 days, especially the ring form.

The results also showed that lead caused a significant decrease (P ≤0.05) in mitotic index parameter whereas the treatment with antioxidant caused decreased chromosomal aberration number and increase number of mitotic index during 28 days.

Histopathological examination for exposure groups of brain showed enlargement, mitotic of nucleus, vaculation, infiltration of lymphocytes, necrosis,degeneration of neurons cells ,odema, hemorrhage and inflammation.

While the liver revealed necrosis, dilation of sinus, hemolytic of nucleus, hyperpigmentation, advanced vaculation of hepatic cells, clear blood engorgement of portal vein, karyorrhexis &karyolysis of many nucleus.The kidney examination showed degeneration of tubules, dilatation of Bowman's space, necrosis, vaculation of cytoplasm, closing of proximal tubules hyperplasia, hemorrhage with degeneration, shrinkage and hyperpigmentation.

Histopathological examination of lung showed hyperpigmentation, death of tissue, hemorrhage, degeneration, fibrosis, odema, lymphocyte infiltration, congestion and necrosis.

The increase in the immune response during treatment with antioxidants (selenium and olive oil) showed some degrees of improvement in brain, liver, kidney and lung tissues.

Main Subjects

Pharmacy, Health & Medical Sciences

No. of Pages

145

Table of Contents

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Language

English

Data Type

Arab Theses

Record ID

BIM-743619