Molecular characterization of field isolates of avian infectious laryngeotracheitis virus from different farms in Iraq
Other Title(s)
التوصيف الجزيئي للعزلات الحقلية لفايروس التهاب الحنجرة و الرغامي المعدي الطيري من حقول مختلفة في العراق
Joint Authors
Odisho, Shony Mikhail
Abbas, Amir Abd al-Amir
Taha, Zaid Haddam
Allawi, Aidah Bara
Source
Iraqi Journal of Veterinary Medicine
Issue
Vol. 40, Issue 2 (31 Dec. 2016), pp.31-35, 5 p.
Publisher
University of Baghdad College of Veterinary Medicine
Publication Date
2016-12-31
Country of Publication
Iraq
No. of Pages
5
Main Subjects
Topics
- Poultry
- Virus diseases
- Iraq
- Inflammation
- Polymerase chain reaction
- Diagnosis
- Molecular aspects
- Glycoproteins
- Throat
Abstract EN
This study was conducted to detect virulent isolates of avian infectious laryngeotracheitis virus in Iraq by real time-polymerase chain reaction with the amplification of glycoprotein g gene which is responsible for virulence of the virus.
Seventy samples (larynx and trachea) were collected from different farms in Iraq to investigate presence of avian infectious laryngeotracheitis virus (detection of virulent isolates from other vaccine strains).
Five samples out of seventy samples were virulent isolates (positive result) by using real time-polymerase chain reaction utilizing flurescein amidite labeled probe specific for detection of isolates that have g gene (by amplification of G gene) for the first time in Iraq.
These virulent isolates were negative by using real time-polymerase chain reaction utilizing quasar-labeled probe specific for the detection of attenuated isolates that lack G gene and targeted a region within glycoprotein J downstream from the sequence of glycoprotein G.
American Psychological Association (APA)
Allawi, Aidah Bara& Abbas, Amir Abd al-Amir& Taha, Zaid Haddam& Odisho, Shony Mikhail. 2016. Molecular characterization of field isolates of avian infectious laryngeotracheitis virus from different farms in Iraq. Iraqi Journal of Veterinary Medicine،Vol. 40, no. 2, pp.31-35.
https://search.emarefa.net/detail/BIM-745774
Modern Language Association (MLA)
Allawi, Aidah Bara…[et al.]. Molecular characterization of field isolates of avian infectious laryngeotracheitis virus from different farms in Iraq. Iraqi Journal of Veterinary Medicine Vol. 40, no. 2 (2016), pp.31-35.
https://search.emarefa.net/detail/BIM-745774
American Medical Association (AMA)
Allawi, Aidah Bara& Abbas, Amir Abd al-Amir& Taha, Zaid Haddam& Odisho, Shony Mikhail. Molecular characterization of field isolates of avian infectious laryngeotracheitis virus from different farms in Iraq. Iraqi Journal of Veterinary Medicine. 2016. Vol. 40, no. 2, pp.31-35.
https://search.emarefa.net/detail/BIM-745774
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 33-34
Record ID
BIM-745774