Spectrophotometric determination of sulfathiazole using 2,4-dinitrophenylhydrazine as coupling reagent

Abstract EN

A rapid, sensitive and selective spectrophotometric method was developed for determination of sulfathiazole (STHZ) in aqueous solution.

The method is based on the oxidative coupling reaction with 2,4-dinitrophenylhydrazine (2,4-DNPH) in a basic medium (pH 10.9) in the presence of potassium periodate to produce an intense orange colour, soluble in water , stable product and absorbs at 492 nm.

Beer's law was in the linear range 2.0-28.0 μg/ml of sulfathiazole, the molar absorptivity, Sandellʼs sensitivity index and detection limit were 1.1437 ×104 liter.

mol-1.cm-1,0.0223 μg.cm-2 and 0.1274 μg/ml respectively.

The RSD value was 0.75 - 1.12 % depending on the concentration.

This method was applied successfully to the determination of sulfathiazole veterinary injection liquid solution (bio prime) with average recovery of not less than 100.38 % .