Spectrophotometric determination of sulfathiazole using 2,4-dinitrophenylhydrazine as coupling reagent
Other Title(s)
التقدير الطيفي للسلفاثايازول باستعمال كاشف الاقتران -4,2 ثنائي نيتروفنيل هيدرازين
Joint Authors
Hamidi, Isra Talib
Ali, Ismail Muhsin
Source
Ibn al-Haitham Journal for Pure and Applied Science
Issue
Vol. 29, Issue 2 (31 Aug. 2016), pp.227-239, 13 p.
Publisher
University of Baghdad College of Education for Pure Science / Ibn al-Haitham
Publication Date
2016-08-31
Country of Publication
Iraq
No. of Pages
13
Main Subjects
Topics
Abstract EN
A rapid, sensitive and selective spectrophotometric method was developed for determination of sulfathiazole (STHZ) in aqueous solution.
The method is based on the oxidative coupling reaction with 2,4-dinitrophenylhydrazine (2,4-DNPH) in a basic medium (pH 10.9) in the presence of potassium periodate to produce an intense orange colour, soluble in water , stable product and absorbs at 492 nm.
Beer's law was in the linear range 2.0-28.0 μg/ml of sulfathiazole, the molar absorptivity, Sandellʼs sensitivity index and detection limit were 1.1437 ×104 liter.
mol-1.cm-1,0.0223 μg.cm-2 and 0.1274 μg/ml respectively.
The RSD value was 0.75 - 1.12 % depending on the concentration.
This method was applied successfully to the determination of sulfathiazole veterinary injection liquid solution (bio prime) with average recovery of not less than 100.38 % .
American Psychological Association (APA)
Hamidi, Isra Talib& Ali, Ismail Muhsin. 2016. Spectrophotometric determination of sulfathiazole using 2,4-dinitrophenylhydrazine as coupling reagent. Ibn al-Haitham Journal for Pure and Applied Science،Vol. 29, no. 2, pp.227-239.
https://search.emarefa.net/detail/BIM-745930
Modern Language Association (MLA)
Hamidi, Isra Talib& Ali, Ismail Muhsin. Spectrophotometric determination of sulfathiazole using 2,4-dinitrophenylhydrazine as coupling reagent. Ibn al-Haitham Journal for Pure and Applied Science Vol. 29, no. 2 (2016), pp.227-239.
https://search.emarefa.net/detail/BIM-745930
American Medical Association (AMA)
Hamidi, Isra Talib& Ali, Ismail Muhsin. Spectrophotometric determination of sulfathiazole using 2,4-dinitrophenylhydrazine as coupling reagent. Ibn al-Haitham Journal for Pure and Applied Science. 2016. Vol. 29, no. 2, pp.227-239.
https://search.emarefa.net/detail/BIM-745930
Data Type
Journal Articles
Language
English
Notes
Includes appendices : p. 233-238
Record ID
BIM-745930