Detection of mecA gene, vanA gene in Staphylococcus Aureus and Staphylococcus Epidermidis from clinical samples

Abstract EN

Current study aimed to identify S.

aureus and S.

epidermidis strains, and investigate of methicillin resistant gene mecA and vancomycin vanA gene in these bacteria and detection the antimicrobial resistance.

The samples were obtained from three hospitals: Al-Mouwasat, Children, and Dermal Venereal Diseases between August 2013 through June 2014.

Forty six strains of S.

aureus and S.

epidermidis were detected, PCR product appeared a single DNA band for nuc, mecA and vanA genes, which coded areas equal to nearly 270 bp, 533 bp, and 1100 bp respectively.

The percentage of presence of mecA gene in S.

aureus strains (MRSA) was 15% and vanA gene (VRSA) was 2.5%.

The percentages of antibiotic resistance were: AMC (65%), AM (70%), AX (55%), GN (30%), CRO (62.5%), PE (7.5%), OFX (7.5%) and VA (2.5%).

The MIC of S.

aureus was ranging between 16 – 32 μg ml-1, except resistant strains was >256 μg ml-1 while MIC for vancomycin was ranging between 4 – 8 μg ml-1, except resistant strains was >256 μg ml-1.

Whereas the MIC for methicillin of all strains of S.

epidermidis was ranging between 8 – 16 μg ml-1.