Determination the causative strain for hydatid cyst in Iraqi cattle by using ND1 gene
Other Title(s)
تحديد السلالة المسببة للأكياس المائية في الأبقار العراقية باستخدام جين ND1
Joint Authors
Daghash, Muhammad Tafash
Muhaidi, Muhammad Jubayr
Ahmad, Maysam Naji
Source
Iraqi Journal of Veterinary Medicine
Issue
Vol. 41, Issue 1 (30 Jun. 2017), pp.11-16, 6 p.
Publisher
University of Baghdad College of Veterinary Medicine
Publication Date
2017-06-30
Country of Publication
Iraq
No. of Pages
6
Main Subjects
Abstract EN
Hydatid Cysts were obtained from 15 cows from liver, lung, spleen, heart, and peritoneal cavity, between December 2014 and October 2015.
Hydatid cysts (protoscoleces) were used for deoxyribonucleic acid extraction by using mechanical grinder.
The purification of mtDNA was done by (promega kit, USA).
The mitochondrial NADH dehydrogenase subunit 1 (ND1) genes were used as targets for polymerase chain reaction amplification, all hydatid cysts yielded amplification products.
Polymerase chain reaction product for NADH1 800 basic pair.
The polymerase chain reaction products were purified and partial sequences were generated.
The sequences obtained were found to align with corresponding region for ND1 gene in the Gene Bank nucleotide database confirming to genotype of sheep strain (G1) in Iraq, Phylogenetic analysis of partial sequence data from ND1 genes for obtained Phylogenetic tree.
G1 genotype was the most common taxon and was the actual source of infection of Iraqi's cattle.
All of 15 strains were G1 genotype (sheep strain) based on the partial sequences of NADH dehydrogenase 1 (ND1).
American Psychological Association (APA)
Muhaidi, Muhammad Jubayr& Ahmad, Maysam Naji& Daghash, Muhammad Tafash. 2017. Determination the causative strain for hydatid cyst in Iraqi cattle by using ND1 gene. Iraqi Journal of Veterinary Medicine،Vol. 41, no. 1, pp.11-16.
https://search.emarefa.net/detail/BIM-747310
Modern Language Association (MLA)
Daghash, Muhammad Tafash…[et al.]. Determination the causative strain for hydatid cyst in Iraqi cattle by using ND1 gene. Iraqi Journal of Veterinary Medicine Vol. 41, no. 1 (2017), pp.11-16.
https://search.emarefa.net/detail/BIM-747310
American Medical Association (AMA)
Muhaidi, Muhammad Jubayr& Ahmad, Maysam Naji& Daghash, Muhammad Tafash. Determination the causative strain for hydatid cyst in Iraqi cattle by using ND1 gene. Iraqi Journal of Veterinary Medicine. 2017. Vol. 41, no. 1, pp.11-16.
https://search.emarefa.net/detail/BIM-747310
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 14-16
Record ID
BIM-747310