Analysis of chemical and biochemical composition of fertile and sterile hydatid cyst fluids of human origin

Abstract EN

The present study was made for the analysis of major chemical and biochemical components of twenty hydatid cysts fluids including two groups (10 fertile cysts and 10 sterile cysts) obtained from the lung and liver of 29 infected patients admitted to Medicine City Hospital for operation during a period of 6 month.

These components were measured by colorimetric assay kits (Biolabo®, Human).

Results revealed marked (P<0.05).differences in the mean values, particularly among enzymes (Glutanicoxaloacetic transaminase, Glutanic pyruvic transaminase and Alkaline phosphatase), between fertile and sterile fluids while the Lactate dehydrogenase level exhibited high activities in fertile and sterile isolates.

These values were less than the normal values in serum.

The mean values of Iron, Zinc Sodium Phosphorus and potassium were found higher in fertile isolates, while a marked decrease accured in sterile isolates.

Furthermore, the Copper revealed quantitative differences (P<0.05) between the two isolates.

Manganese and chloride constituted the major volume in the fluid anions, in fertile and reached the maximum in sterile fluid.

Differences were detected in the levels of glucose of fertile which increased significantly (P<0.05).

The data detected no significant difference (P>0.05) in total protein which exhibited high in fertile and sterile fluid.

Also Calcium and Magnesium mean values revealed the same in both isolates.

Trace elements like Urea, Uric acid, Bilirubin Creatinine and Lipid increase in liver diseases such as the Cholesterol, and Triglycerides level which was found higher in fertile and sterile isolates.

In comparison with previous studies the present investigations reflected quantitative difference in mean values of biochemical parameters of fertile and sterile of hydatid cyst fluids contributed to fertility of cyst and viability of protoscoles.

Fertile cyst which contained broad capsule and protoscoles responsible of higher concentration in these elements than sterile fluid which lack the broad capsule and protoscoles, which may point to some variable in parasite metabolism endurance, growth rate, intermediate host specificity location of cyst among lungs or livers or even strain variation.