Effect of apple juice daily supplementation on male mice fertility

Abstract EN

This study was carried out to examine the effect of apple (Malus domestica) juice daily supplementation on male mice fertility.

Sixteen healthy male mice (Mus musculus), in the age of 90 ±10 days and the weight of 31 ± 2.4 g, were selected from Swiss albino strain then randomly separated into two groups, each group contained 8 male mice.

The males of the first group (C) were given daily gavage administration of 0.1 ml of tap water for 30 days .The males of the second group (T) were given daily gavage administration of 0.1 ml of apples juice for 30 days.

After 24 hours of the last administration, the animals were euthanized, the sperms parameters calculated to assess the sperms’ progressive motility, concentration, vitality and morphology, and a micrometry study for the testes was conducted to evaluate both of the diameters of seminiferous tubules and the thicknesses of germinal cell layers then the recorded data was statistically analyzed.

According to the outcomes of the statistical analysis, the sperms progressive motility increased (P ≤0.01) from 72 ± 3 % (control group) to 88 ±2 %(treatment group); the sperms concentration improved (P ≤0.01) from 1.9± 0.2 X106/ml (control group) to 3.5±0.2 X106/ml(treatment group); the percentage of normal sperms raised (P≤0.05) from 89.7±3% (control group) to 94.6±1 % (treatment group); also there is non significant improvement (P≥0.05) in the vital sperms percentages which increased from 89.4±7% (control group) to 90.1±5% (treatment group); the diameters of seminiferous tubules which increased from 163.2±27μm (control group) to 168.5±19μm (treatment group) and the thicknesses of germ cell layers which increased from 51.9±12μm (control group) to 56.8±9μm (treatment group).

From the outcomes of our recent study we can conclude that apple juice daily supplementation had positive effect on male mice fertility.