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Detection of Bacillus cereus by polymerase chain reaction (PCR)
Other Title(s)
الكشف عن بكتريا التسمم الغذائي Bacillus cereus بواسطة تفاعل أنزيم البلمرة المتسلسل
Joint Authors
Nasir, Raji Tumah
Tahir, Isra Zahir
Salih, Manal Badi
Source
Journal of Thi-Qar University for Agricultural Researches
Issue
Vol. 4, Issue 1 (30 Jun. 2015), pp.30-40, 11 p.
Publisher
University of Thi-Qar College of Agricultural and Marshes
Publication Date
2015-06-30
Country of Publication
Iraq
No. of Pages
11
Main Subjects
Topics
Abstract EN
In this study we used a reliable, rapid and sensitive method for the identification of the B.
cereus group spp.
A method is required to detect a small amount of bacterial DNA in a sample is desirable and necessary.
In this study we collect 300 sample from different source(stool ,rice,potato ,clinical material) in Thi- Qar province and used BC1 gene as specific gene to detect Bacillus cereus from other species .
The gene was investigated in highest rate of isolates(95%), PCR was the first nucleic acid-based assay for detecting pathogens.
The method is sensitive, specific and allows small amount of bacterial DNA in a sample to be detected .The present PCR assay is the first molecular tool for rapid detection of Bacillus cereus.
American Psychological Association (APA)
Nasir, Raji Tumah& Salih, Manal Badi& Tahir, Isra Zahir. 2015. Detection of Bacillus cereus by polymerase chain reaction (PCR). Journal of Thi-Qar University for Agricultural Researches،Vol. 4, no. 1, pp.30-40.
https://search.emarefa.net/detail/BIM-747352
Modern Language Association (MLA)
Nasir, Raji Tumah…[et al.]. Detection of Bacillus cereus by polymerase chain reaction (PCR). Journal of Thi-Qar University for Agricultural Researches Vol. 4, no. 1 (2015), pp.30-40.
https://search.emarefa.net/detail/BIM-747352
American Medical Association (AMA)
Nasir, Raji Tumah& Salih, Manal Badi& Tahir, Isra Zahir. Detection of Bacillus cereus by polymerase chain reaction (PCR). Journal of Thi-Qar University for Agricultural Researches. 2015. Vol. 4, no. 1, pp.30-40.
https://search.emarefa.net/detail/BIM-747352
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 36-39
Record ID
BIM-747352