Development of a RP-HPLC method for evaluationof in vitro permeability of voriconazole in the presence of enhancers through rat skin
Joint Authors
Madhavi, G.
Krishna, M. Vamsi
Dash, Rajendra N.
Venugopal, P.
Reddy, B. Jalachandra
Sandeep, P.
Source
Journal of Saudi Chemical Society
Issue
Vol. 21, Issue 1 (31 Jan. 2017), pp.1-10, 10 p.
Publisher
Publication Date
2017-01-31
Country of Publication
Saudi Arabia
No. of Pages
10
Main Subjects
Abstract EN
An isocratic RP-HPLC-UV method for the analysis of voriconazole in skin diffusate samples has been developed and validated.
Experimental design was employed to optimize the method.
The method was validated as per ICH guidelines.
Linearity was observed over the concentration range of 2-100 igmL^1 (r2 = 0.999).
Limits of detection and quantification were 0.6 and 2 igmL^1, respectively.
Intra-day and inter-day precision (% RSD) was within the ICH limits (62%).
The method was successfully used to analyze skin diffusate samples, and the effectiveness of different permeation enhancers was compared with respect to flux and permeability coefficient.
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This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
American Psychological Association (APA)
Krishna, M. Vamsi& Dash, Rajendra N.& Venugopal, P.& Reddy, B. Jalachandra& Sandeep, P.& Madhavi, G.. 2017. Development of a RP-HPLC method for evaluationof in vitro permeability of voriconazole in the presence of enhancers through rat skin. Journal of Saudi Chemical Society،Vol. 21, no. 1, pp.1-10.
https://search.emarefa.net/detail/BIM-747645
Modern Language Association (MLA)
Krishna, M. Vamsi…[et al.]. Development of a RP-HPLC method for evaluationof in vitro permeability of voriconazole in the presence of enhancers through rat skin. Journal of Saudi Chemical Society Vol. 21, no. 1 (Jan. 2017), pp.1-10.
https://search.emarefa.net/detail/BIM-747645
American Medical Association (AMA)
Krishna, M. Vamsi& Dash, Rajendra N.& Venugopal, P.& Reddy, B. Jalachandra& Sandeep, P.& Madhavi, G.. Development of a RP-HPLC method for evaluationof in vitro permeability of voriconazole in the presence of enhancers through rat skin. Journal of Saudi Chemical Society. 2017. Vol. 21, no. 1, pp.1-10.
https://search.emarefa.net/detail/BIM-747645
Data Type
Journal Articles
Language
English
Notes
Includes bibliographical references : p. 65-66
Record ID
BIM-747645