تحديد الباراسيتامول و الإيبوبروفن باستخدام الكروماتوغرافيا السائلة عالية الأداء

Abstract EN

The purpose of this article is how to determine simultaneously the content of paracetamol and ibuprofen in pure forms and in pharmaceutical preparations (tablets and suspension dosage forms) by high performance liquid chromatography (HPLC).

Analysis was conducted on a C18 -column (3.9 mm x150mm, 5 µm) with mobile phase consisting of methanol – water – phosphoric acid in ratios [75: 24.7 :0.3] respectively, and flow rate 0.8 ml/min.

An ultraviolet detector was used at wavelength of 214 nm.

Results were linear in the range of 2.5000 -80.000 µg/ml for paracetamol and 3.125-100.000 µg/ml for ibuprofen.

The correlation coefficients were R= 0.999969 and R= 0.999993 for paracetamol and ibuprofen respectively.

The relative standard deviations (n = 6) of intraday assay and interday assay were 0.350%, 0.699% for paracetamol and 0.152%, 0.382% for ibuprofen, respectively.

This method was characterized by high accuracy, precision and suitable for quality control in pharmaceutical industries.